FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004689571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1732729
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCAGCGGCGGTCACAGAGGTCAGCCTCAGGGAGACCTGATTCTTCGACGTGTCGGCGGA55080.3178800608750705No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT53820.31060829477662116No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44790.25849397107106764No Hit
GAGTTACAGTATCCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT43590.25156847954873496No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39800.22969546882403422No Hit
GTCTCTGGTGTCTCCATCACCAGTAGAAGTCACTTGTGGGGCTGGATCCG37800.21815298295347973No Hit
GATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA36740.21203546544208587No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31170.17988964229259163No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAATCTGTC31080.17937023042841668No Hit
GTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTATCCCTCGAACGTTCGGCCAAGGGA30830.17792741969459736No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30640.1768308835368947No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCACCTATTTAAATTGGTATCAACAGAAA30350.17515722308566428No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30130.1738875496399033No Hit
GTGTATTACTGTGCGAGACAAGCATCAAATTTTGGCCCCAACTGGTTCGA28950.16707748297627614No Hit
CCATAGATCAGGACATAAGGGGCTTTTCCTGGTTTCTGTTGATACCAATT28920.16690434568821785No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27950.16130624004099892No Hit
CAGTAATACACAGCCGTGTCAGCGGCGGTCACAGAGGTCAGCCTCAGGGA27440.1583629061440075No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA27220.15709323269824654No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25810.1489557801595056No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25230.1456084592570448No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25100.14485819767545877No Hit
CACCTGCACTGTCTCTGGTGTCTCCATCACCAGTAGAAGTCACTTGTGGG24810.14318453722422836No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24740.14278055021875896No Hit
CCCTGAGGCTGACCTCTGTGACCGCCGCTGACACGGCTGTGTATTACTGTGCGAGACAAGCATCAAATTTTGGCC23190.13383512366907924No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23180.13377741123972645No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22900.13216146321784883No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22840.1318151886417322No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22830.13175747621237943No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22590.1303723779079129No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22580.13031466547856013No Hit
GTATTACTGTGCGAGACAAGCATCAAATTTTGGCCCCAACTGGTTCGACCCGTGGGGCCAGGGAACCCTGGTCAC21760.12558224627163278No Hit
GTCTCCATCCTCCCTGTCTGGATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAC21540.1243125728258718No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG21370.12333146152687466No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21150.12206178808111368No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20810.12009956548311941No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC20530.11848361746124177No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTTCGAGGGATA20490.11825276774383069No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20470.11813734288512515No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT20390.11767564345030296No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20360.11750250616224464No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20240.11680995701001137No Hit
GTCTGGATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA20140.11623283271648364No Hit
ACTTACTACTGTCAACAGAGTTACAGTATCCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA19310.11144270108020353No Hit
GCCTCAGGGAGACCTGATTCTTCGACGTGTCGGCGGACATCGTGACTCGA18980.10953819091156206No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18800.10849936718321214No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18640.10757596831356779No Hit
ACTACAACCCGTCCCTCAAGACTCGAGTCACGATGTCCGCCGACACGTCGAAGAATCAGGTCTCCCTGAGGCTGA18590.10728740616680393No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18550.10705655644939283No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18420.1063062948678068No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTT18290.10555603328622075No Hit
GGACATAAGGGGCTTTTCCTGGTTTCTGTTGATACCAATTTAAATAGGTG18070.10428635984045975No Hit
GATTTCACTCTCACCATCAGCAGTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTATC17860.10307439882405155No Hit
CTTATGTCCTGATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGA17720.10226642481311274No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA17380.10030420221511846No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17330.10001564006835462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)