FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1609464
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATAATCGGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG55440.34446250428714154No Hit
GTAATATATAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA54730.3400510977567687No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC54070.33595035365811227No Hit
GATTATCACTGTGCAACATGGGATGACAACCTGAGTGGATGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA47890.2975524770979655No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45890.2851259798293096No Hit
ATATATTACTGTGCGAGACAGACGAGTATTGTAGCTCCTCATGAAGTCTA44040.27363146985580294No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA42260.26257188728669917No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT39690.24660383829647634No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC39630.24623104337841667No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39300.24418067132908847No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC37080.2303872593608804No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCATCCACTCAGG33880.21050486373103094No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32630.202738302938121No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG31050.19292137009588284No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT30320.18838569859282345No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG30290.1881993011337936No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC29890.1857140016800624No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT29580.18378789460342077No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29430.18285590730827156No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29310.1821103174721522No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG28740.17856876575058528No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28330.17602133381051083No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27910.17341176938409308No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA27400.17024301258058583No Hit
GTGCAACATGGGATGACAACCTGAGTGGATGGGTGTTCGGCGGAGGGACC26660.16564520859118315No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG26380.16390549897357135No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25590.15899703255245226No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24970.15514481839916894No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC24870.1545234935357361No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC24690.15340510878155708No Hit
ATGTTGCACAGTGATAATCGGCCTCATCCTCGGACCGGAGCCCACTGATG24690.15340510878155708No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC23830.14806171495603504No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA23750.14756465506528882No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23470.145824945447677No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23190.14408523583006516No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATTTCCGTAGACACGTCCAAGAA22540.140046624217752No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC22260.13830691460014016No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22090.1372506623323044No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21950.13638080752349852No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG21870.13588374763275227No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21810.1355109527146926No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21790.13538668774200605No Hit
CTCTTGTTCTGGAGGCACCTCCAACATCGGACCTAATTATGTGTGCTGGTACCAGCAGCTCCCAGGAACGGCCCC21670.1346410979058867No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA21570.13401977304245388No Hit
CCTCCAACATCGGACCTAATTATGTGTGCTGGTACCAGCAGCTCCCAGGA21440.13321205071999126No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCATCCACTCAGGT21370.1327771233155883No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20640.12824145181252888No Hit
CACCTGCACTGTCTCTGGCGCCGCCATCACCGGTAGTAGTCACTACTGGG20310.12619107976320065No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19920.12376791279581276No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19710.12246313058260391No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC19620.12190393820551439No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCCGATTATCACTGTGCAA19370.12035062604693239No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19200.11929437377909664No Hit
CCCCTGAGGGCCGGTGATCATCCCCATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGCACA19160.11904584383372353No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG18940.11767892913417137No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18910.11749253167514154No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG18890.11736826670245498No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18700.11618774946193265No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC18680.11606348448924611No Hit
GTCTCTGGCGCCGCCATCACCGGTAGTAGTCACTACTGGGGCTGGATCCG18530.11513149719409692No Hit
CTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGCATATCTATTATAGTGGGAGCAC18100.1124598002813359No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC18040.11208700536327622No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC18010.11190060790424639No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18010.11190060790424639No Hit
CAGTAATATATAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGA17830.11078222315006735No Hit
ACATGGGATGACAACCTGAGTGGATGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGG17820.11072009066372408No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17790.11053369320469424No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17790.11053369320469424No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17710.110036633313948No Hit
CTATAATAGATATGCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGTGACTACTA17610.10941530845051521No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17190.10680574402409745No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17130.10643294910603779No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16950.10531456435185875No Hit
CCCATCCACTCAGGTTGTCATCCCATGTTGCACAGTGATAATCGGCCTCATCCTCGGACCGGAGCCCACTGATGG16800.10438257705670956No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16790.10432044457036628No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16710.10382338467962005No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16640.10338845727521709No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16570.10295352987081413No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16450.10220794003469479No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT16200.1006546278761128No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16190.1005924953897695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAA16950.052.0782470
AATCGCG507.201743E-848.08011636
GGTATCA10400.045.6281781
GCGAATC2750.039.8754233
CGCGTAT350.001356865739.37587439
GGCGAAT3250.037.93702732
AGGGGGG3400.037.68754670
GTGGTAT2650.037.3597341
CGTATGC2350.036.73604241
TCGTATG2350.036.69681540
CGAATCG754.2487954E-836.5718634
GTATCAA23450.036.104431
ACGAGAC3600.036.02784722
TCTCGTA2300.035.9102138
GACAGGT2900.035.3162771
CGAGACT3750.034.5888823
ATCGCGT501.9000703E-434.38055837
ACTAAGG3700.034.1645527
TATACAC3800.034.125283
TAAGGCG3650.033.72591829
Produced by FastQC (version 0.11.5)