FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608127
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22930.37705939713250686No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19960.328220914381371No Hit
GTTTTCTACAGGTCCAACAATAAGAATTATTTAACTTGGTATCAGCAGAA16170.2658984060895175No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15580.25619648527363525No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15190.24978335117500128No Hit
CTATTATTGTGCGAGAGGTCATCCTGAGAGTTCGTCCTTTTTTGACTACTGGGGCCGGGGATCCCTGGTCACCGT14360.23613488629842125No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT13860.2279129195053007No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12500.2055491698280129No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12310.20242482244662707No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12280.20193150443903987No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12270.20176706510317746No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11660.19173626561557044No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11290.18565201018866126No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11220.18450093483762436No Hit
GTAGAAAACAGTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA11020.18121214812037617No Hit
TTTTAGTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGCACGAACTGTGGCTGCACCATCTGTCTT10880.17890999741830244No Hit
GTTTATTACTGTCAGCAACATTTTAGTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGCACGAA10830.1780878007389904No Hit
CAATAATAGAGGGCCGTGTCCTCGACTCTCAGGGCGGTAAGTTGTAAATA10800.17759448273140316No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10450.1718391059762188No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10340.17003027328173229No Hit
GTACTAAAATGTTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGTTGGTGATGGTGAGAGTGAAAT9960.1637815785189607No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9520.1565462477410146No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9470.15572405106170256No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTCTA9400.1545729757106657No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8760.14404885821547145No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8730.1435555402078842No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8550.14059563216236082No Hit
CACCATCACCAACCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAACATTTTAGTACTCCGTGGACGTT8510.13993787481891118No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT8500.13977343548304877No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGTTGGTGATGGTGAGAGTGA8400.13812904212442467No Hit
ACCTGGGGACTCCCTGAGGCTCTCCTGTGCTCCCTCTGGATTCCCCTTTAGCGGCTTTGCCATGAGTTGGGTCCG8300.13648464876580058No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8300.13648464876580058No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTCTACAGGTCCAA8170.13434693739958922No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8140.133853619392002No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8100.13319586204855235No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGTGAC8070.13270254404096513No Hit
GTGCAGCCACAGTTCGTGCGATTTCCACCTTGGTCCCTTGGCCGAACGTC8070.13270254404096513No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8050.1323736653692403No Hit
GCCACAGTTCGTGCGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGG8040.1322092260333779No Hit
GTCCCTGACCGATTCCGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC8020.13188034736165308No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7860.1292493179878545No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7700.12661828861405594No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7610.12513833459129425No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7520.12365838056853257No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7340.12069847252300918No Hit
GATTTCACTCTCACCATCACCAACCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAACATTTTAGTACT7070.1162586104547241No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6970.11461421709609998No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6820.11214762705816383No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6650.10935215834850286No Hit
ACTGAGGAGACTGACCTGGTTTCTGCTGATACCAAGTTAAATAATTCTTATTGTTGGACCTGTAGAAAACAGT6540.10754332565401635No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC6440.10589893229539225No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG6420.10557005362366743No Hit
CCCTGAGGCTCTCCTGTGCTCCCTCTGGATTCCCCTTTAGCGGCTTTGCC6330.10409009960090573No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6330.10409009960090573No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6260.10293902424986887No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTT6250.10277458491400646No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6200.10195238823469441No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6120.10063687354779512No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6120.10063687354779512No Hit
CTCCTCAGTTGCTCATTTACTGGGCATCTACTCGGGCATCCGGGGTCCCT6110.1004724342119327No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6090.10014355554020789No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)