FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608127
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105231.730395131280144No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22640.3722906563924969No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17150.2820134610040337No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16180.2660628454253799No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15960.2624451800364069No Hit
GTTTTCTACAGGTCCAACAATAAGAATTATTTAACTTGGTATCAGCAGAA14580.23975255168739423No Hit
CTATTATTGTGCGAGAGGTCATCCTGAGAGTTCGTCCTTTTTTGACTACTGGGGCCGGGGATCCCTGGTCACCGT13690.22511745079563972No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12500.2055491698280129No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11780.19370953764591936No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11170.18367873815831232No Hit
GTACTAAAATGTTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGTTGGTGATGGTGAGAGTGAAAT11000.18088326944865135No Hit
TTTTAGTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGCACGAACTGTGGCTGCACCATCTGTCTT10630.17479901402174217No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10590.17414125667829253No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10550.1734834993348429No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10550.1734834993348429No Hit
GTTTATTACTGTCAGCAACATTTTAGTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGCACGAA10520.17299018132725566No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10160.16707036523620888No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10040.16509709320585997No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9950.16361713918309825No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9590.15769732309205148No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGTTGGTGATGGTGAGAGTGA9350.15375077903135365No Hit
CAATAATAGAGGGCCGTGTCCTCGACTCTCAGGGCGGTAAGTTGTAAATA9320.15325746102376642No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8860.14569325157409554No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8700.14306222220029696No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGTGAC8630.1419111468492601No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTCTA8520.1401023141547736No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8470.13928011747546154No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8280.13615577009407576No Hit
ACCTGGGGACTCCCTGAGGCTCTCCTGTGCTCCCTCTGGATTCCCCTTTAGCGGCTTTGCCATGAGTTGGGTCCG8230.1353335734147637No Hit
GTAGAAAACAGTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA8140.133853619392002No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT8090.13303142271268995No Hit
GCCACAGTTCGTGCGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGG8070.13270254404096513No Hit
CACCATCACCAACCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAACATTTTAGTACTCCGTGGACGTT8060.13253810470510272No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8000.13155146868992826No Hit
GTGCAGCCACAGTTCGTGCGATTTCCACCTTGGTCCCTTGGCCGAACGTC7940.13056483267475377No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7720.1269471672857808No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7600.12497389525543184No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7560.12431613791198219No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7500.12332950189680775No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7400.12168510853818362No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7360.121027351194734No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7240.11905407916438507No Hit
GTCCCTGACCGATTCCGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC7170.1179030038133482No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7070.1162586104547241No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTCTACAGGTCCAA7040.11576529244713687No Hit
GTCCCAGACCCGCTGCCACGGAATCGGTCAGGGACCCCGGATGCCCGAGT6980.1147786564319624No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6910.11362758108092552No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6780.1114898697147142No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6720.11050323369953974No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6710.11033879436367733No Hit
GATTTCACTCTCACCATCACCAACCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAACATTTTAGTACT6590.1083655223333284No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6340.10425453893676816No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACGGAATCGGTCAGGGACCCCG6330.10409009960090573No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6230.10244570624228164No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTT6200.10195238823469441No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6090.10014355554020789No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)