FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407111
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65861.617740616195583No Hit
GTCTTATATAGTTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAAAA34670.8516104944351786No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13990.3436409234827848No Hit
ATATAAGACAGTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA12440.3055677689868365No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9880.2426856557548187No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9460.23236905905269079No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8780.21566599772543607No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA8580.2107533326291847No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8460.2078057335714338No Hit
GTTTATTACTGTCAGCAATATTTTGATATTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA8040.19748913686930591No Hit
ACTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAACTATATAAGACAGT7610.18692690691236544No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7570.18594437389311513No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG7350.18054044228723862No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7190.1766103102102375No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6870.16875004605623528No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6690.164328647469609No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT6570.16138104841185819No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC6500.15966161562817022No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6340.1557314835511691No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6260.15376641751266854No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6130.15057318520010513No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6050.14860811916160457No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACCCCG5910.1451692535942286No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTTTGATATT5770.14173038802685262No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5610.1378002559498515No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5560.13657208967578865No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC5490.1348526568921007No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA5390.13239632434397497No Hit
GTACAAGGCTGTGTCCTCGATTTTCAGGCTGTTCATTTGCAGATACAACG5270.12944872528622414No Hit
GTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACCCCGGATTCCCGGGT5060.1242904269351602No Hit
GCCTTGTACTTCTGCACGACCGGGGGAGTGGTTTGGGGAGTTAAAGTCTA5030.12355352717072247No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACC4990.1225709941514722No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG4910.12060592811297165No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG4860.1193777618389088No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT4810.11814959556484596No Hit
GGCAGGGGCAGCAAGATGGTGTTGCGGACCCAGGTCTTCATTTCTCTGTT4800.11790396231003339No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTCTTATATAGTTCCAC4660.11446509674265741No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4550.11176313093971915No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4460.109552431646406No Hit
GTCCCTGACCGATTCACTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC4450.10930679839159345No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA4350.10685046584346775No Hit
CTACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATC4320.10611356607903005No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT4270.1048853998049672No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT4260.10463976655015464No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG4240.10414850004052949No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTTTGCTGGTA4230.1039028667857169No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4080.10021836796352838No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAAGG100.00747704142.4206470
GCCTATA150.00267050866.8422851
TAAGCAA200.00584889854.8368359
CGAATCA200.007091080352.23123643
GGATTAT200.00833993650.1317141
TGCGTGC200.00835615850.107088
GTGGTAT1250.042.7790641
TGGTATC1250.042.7685472
GACAGGT551.882454E-742.5360031
AGATTTA350.001433217938.93260636
ACGCCCC350.00150156638.56536531
ACAGCTC1150.037.7804221
TCCGAGA1952.0372681E-1036.51811270
GTATCAA7100.035.7747461
GTGAGCG1854.9385562E-934.6428670
TTGCTCA18050.033.92846370
TAGGAAC10350.033.6239321
GACAGGC601.6660944E-533.4211431
AGGAACT10650.033.30927722
AGTTGCT11300.033.0908768
Produced by FastQC (version 0.11.5)