FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804859
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATATAGTTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAAAA153260.8491522052415175No Hit
ATATAAGACAGTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA70970.3932163121883759No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63220.35027666981188005No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52960.2934301239044158No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46550.2579148842097915No Hit
ACTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAACTATATAAGACAGT44210.24494988251159786No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42840.23735926185923664No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42380.23481058631172852No Hit
GTTTATTACTGTCAGCAATATTTTGATATTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA41080.22760780759050986No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT40940.22683212372822475No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA36070.19984940651873637No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35480.19658045309910635No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG34830.19297906373849705No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33590.18610872095825767No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32980.18272895555830124No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32320.1790721602075287No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32300.17896134822720225No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32070.17768701045344815No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC31810.17624645470920441No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC30950.17148153955516748No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30510.16904367598798575No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29970.16605175251917184No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29870.16549769261753963No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28700.15901519176844287No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTTTGATATT28290.1567435461717508No Hit
GTCCCTGACCGATTCACTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC27960.15491514849636454No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA27020.14970698542102182No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG26180.14505288224731128No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25130.13923525328017314No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24500.13574467589989023No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTTTGCTGGTA24380.1350798040179316No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23810.13192166257862803No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT23770.13170003861797513No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23470.1300378589130785No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTCTTATATAGTTCCAC23180.12843108519834515No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22760.12610403361148986No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22550.12494050781806225No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22310.12361076405414495No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22250.12327832811316562No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22210.12305670415251274No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAA21910.12139452444761613No Hit
GGCAGGGGCAGCAAGATGGTGTTGCGGACCCAGGTCTTCATTTCTCTGTT21290.11795935305749647No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC20980.11624176736243662No Hit
ACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGTTGCTCATT20880.11568770746080441No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTTTGATATTCCGTGGACGTT20760.11502283557884577No Hit
CTACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATC20720.1148012116181929No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20580.11402552775590781No Hit
GTACAAGGCTGTGTCCTCGATTTTCAGGCTGTTCATTTGCAGATACAACG20300.11247416003133763No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20290.1124187540411744No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20240.1121417240903583No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTTTGATATTCCGT19880.11014710844448236No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT19500.10804168081827999No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACC19470.10787546284779033No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19120.1059362531920776No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC19090.10577003522158794No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18790.10410785551669133No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTCTTATATAGTTCCACCAATAGGAACTA18730.103775419575712No Hit
TTATTACTGTCAGCAATATTTTGATATTCCGTGGACGTTCGGCCAAGGGA18700.10360920160522236No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18490.10244567581179471No Hit
GGATCTCTGGTGCCTCCGGGGATACCGTTATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG18450.10222405185114182No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18280.10128215001836709No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC18120.10039565417575555No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT400.00843956949.98636670
GGTATCA11050.043.6350171
CGTATGC2050.035.3843741
GACCGGA951.6879517E-435.07814870
GTGGTAT2900.034.196241
GTATCAA24600.033.918221
TCGTATG2150.033.706540
GACAGGT3100.033.0931361
GTATGCC2250.032.27614642
TATACAC2550.030.8257243
CTGGAGA24200.030.52247869
CTTATAC2700.030.3966561
CGTATCG450.00484086430.37981413
CTATACA450.0048415230.3789714
TGGTATC3300.030.0337562
TTTCGAT1353.8059796E-529.6215570
TATCAAC28400.029.4831142
TCTGGAG23600.029.37099368
ATCAACG28950.029.041043
TCAACGC29300.028.6941344
Produced by FastQC (version 0.11.5)