FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804859
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATATAGTTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAAAA138720.7685918955441948No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63940.35426590110363193No Hit
ATATAAGACAGTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA53920.298749098960085No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44380.2458917843443726No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43940.24345392077719089No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42740.23680520195760446No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40920.22672131174789834No Hit
GTTTATTACTGTCAGCAATATTTTGATATTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA40760.22583481590528678No Hit
ACTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAACTATATAAGACAGT40760.22583481590528678No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA39800.22051584084961762No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT37790.20937923682681028No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG34420.19070741814180497No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33620.18627493892874736No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32780.18162083575503682No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32690.18112218184356785No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31450.1742518390633285No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC30050.1664950004404776No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC29800.1651098506863971No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28640.15868275582746352No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28450.15763004201436234No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28310.15685435815207724No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28080.1555800203783232No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA27560.1526989088898357No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27500.15236647294885639No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTTTGATATT26860.1488204895784103No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26140.1448312582866584No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25890.1434461085325779No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25550.1415623048670284No Hit
GTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACCCCGGATTCCCGGGT25500.1412852749162123No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25410.1407866210047433No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25240.13984471917196856No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACCCCG25230.13978931318180532No Hit
GTCCCTGACCGATTCACTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC25100.13906903530968348No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT24600.13629873580152244No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23560.1305365128245475No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACC23550.1304811068343843No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23170.12837567920818194No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22660.12554997370985765No Hit
GGCAGGGGCAGCAAGATGGTGTTGCGGACCCAGGTCTTCATTTCTCTGTT22000.12189317835908511No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21950.12161614840826901No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTTTGCTGGTA21700.1202309986541885No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21350.11829178899847578No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTCTTATATAGTTCCAC20730.11485661760835611No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20680.11457958765754002No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTTTGATATTCCGT20650.11441336968705035No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20650.11441336968705035No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20240.1121417240903583No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20150.11164307017888932No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20070.11119982225758354No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19830.10987007849366627No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19720.10926061260187084No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19440.10770924487730066No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTTTGATATTCCGTGGACGTT19100.10582544121175115No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAA18920.10482813338881321No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT18810.10421866749701778No Hit
GTACAAGGCTGTGTCCTCGATTTTCAGGCTGTTCATTTGCAGATACAACG18770.10399704353636488No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18620.1031659536839166No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGAATATCAAAATATTGCTGACAGTAATAAACTGCCACATCTTCAGC18430.10211323987081539No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18400.10194702190032573No Hit
GGATCTCTGGTGCCTCCGGGGATACCGTTATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG18090.1002294362052659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12600.045.2356451
GTGGTAT3250.040.9558641
TGGTATC3300.040.331972
GACAGGT3100.037.432781
GTCTAAT400.002737747534.129891
GTACGGA601.4459098E-534.129891
GTATCAA28000.033.0328561
CTGGAGA19600.031.03945269
CCGCTAT900.004400918730.97561570
AATGGTA4750.029.34532270
CGGTCCG600.00610593728.97015669
TCTGGAG19800.028.3405968
ATCAACG31600.028.2920783
TCAACGC31850.027.9628664
TATCAAC32600.027.8459972
TAGGAAC45750.027.6291221
CAACGCA32350.027.5306745
AACGCAG32650.027.4867366
GTCTGGA19950.027.2606167
ACTACTT46650.027.18515426
Produced by FastQC (version 0.11.5)