FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1494601
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTCTACTGGTCCAACAGTAAGAATTATTTGACTTGGTATCAGCAGAA64280.43008133943440424No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60730.4063291808315396No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56530.37822803544223504No Hit
TTTCAGTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGCACGAACTGTGGCTGCACCATCTGTCTT41320.27646174463953926No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39280.2628126168790199No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38740.2591996124718236No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37870.2533786609268962No Hit
GTTTATTACTGTCAGCAACATTTCAGTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGCACGAA34900.23350713668731654No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32600.2181184142122212No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32560.21785078425613258No Hit
GTAGAAAACAGTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA32300.2161111895415566No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31580.21129385033196152No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31550.21109312786489506No Hit
GTACTGAAATGTTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGTTGGTGATGGTGAGCGTGAAAT31500.2107585904197843No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30910.20681104856747723No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30490.20400093402854674No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT30430.2035994890944138No Hit
CACCATCACCAACCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAACATTTCAGTACTCCGTGGACGTT30150.20172607940179352No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29240.19563749790077753No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27910.18673880186083108No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26350.1763012335733751No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTCTA24850.16626511022005205No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGTTGGTGATGGTGAGCGTGA24540.16419097806036526No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24480.16378953312623235No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTCTACTGGTCCAA24350.16291973576894436No Hit
GCCACAGTTCGTGCGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGG23850.15957436131783667No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGCGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGAGTA23780.1591060088946816No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG23220.15535918950944097No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGT22670.15167927761322253No Hit
GTGCAGCCACAGTTCGTGCGATTTCCACCTTGGTCCCTTGGCCGAACGTC21850.1461928635134059No Hit
ATATTATTGTACGAGAGGTCATCCTGAGAGTTCGTCCTTTTTTGACTCCTGGGGCCGGGGAACCCTGGTCACCGT21710.14525615866709576No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21600.14452017628785208No Hit
GTCCCTGACCGATTCCGTGGCAGCGGGTCTGGGACAGATTTCACGCTCAC21530.144051823864697No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTCTGGGCTGAGGTGGCTTTTT21360.14291439655132038No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21210.14191078421598807No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21020.14063954192456715No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20730.1386992247429247No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20490.137093445006393No Hit
TCAGTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGCACGA20100.13448405293452903No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20090.13441714544550684No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT19930.1333466256211524No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19420.12993434368102258No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19330.12933217627982319No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC18920.12658896922991486No Hit
ACTGAGGAGACTGACCTGGTTTCTGCTGATACCAAGTCAAATAATTCTTACTGTTGGACCAGTAGAAAACAGT18520.12391266966902872No Hit
GTCAGGGACCCCGGATGCCCGGGTAGATGCCCAGTAAATGAGCAACTGAGGAGACTGACCTGGTTTCTGCTGATA18500.12377885469098442No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18270.12223998244347489No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTT17860.11949677539356658No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17760.11882770050334504No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17350.11608449345343673No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT17230.11528160358517088No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17140.11467943618397151No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT17110.11447871371690505No Hit
GAGTACTGAAATGTTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGTTGGTGATGGTGAGCGTGAAAT17110.11447871371690505No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17040.11401036129374997No Hit
GACCGATTCCGTGGCAGCGGGTCTGGGACAGATTTCACGCTCACCATCAC16900.11307365644743983No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGTTGGTGATGGTGAGCGTGAAATCTGTCCCAGACCCGCTGCCA16660.11146787671090813No Hit
TCACCAACCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAACAT16650.11140096922188597No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16640.11133406173286382No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16520.11053117186459799No Hit
GGATCTCTGGTGTCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG16520.11053117186459799No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16520.11053117186459799No Hit
GGTCTGGGACAGATTTCACGCTCACCATCACCAACCTGCAGGCTGAAGAT16320.1091930220841549No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16250.10872466966099982No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16130.10792177979273399No Hit
CAATAATATAGGGCCGTGTCCTCGACTCTCAGGGCGGTTAGTTCTAAATA16020.10718579741349028No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15900.10638290754522443No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15890.10631600005620229No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15660.10477712780869275No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15590.10430877538553768No Hit
CTCCAGTCCCTTCCCTGGAGTCTGGCGGACCCAACTCATGGCAAAGCCGC15580.10424186789651552No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15530.10390733045140477No Hit
ATATAGGGCCGTGTCCTCGACTCTCAGGGCGGTTAGTTCTAAATACAAAA15250.10203392075878445No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15150.10136484586856293No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15110.10109721591247431No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT451.5261976E-461.3211370
TATAGCG200.0059217654.67342464
TATATCG453.2196112E-853.25321636
ATTCGCC200.00736622351.73793841
CGCGTAT200.00745192451.58679639
ATATCGT408.6451837E-751.41563837
TATGTCG200.007587949351.3513136
CGTATGC3900.049.52691741
ATGCCGT4150.048.4250544
TCGTATG3950.047.954940
TATCTCG3300.047.72041736
GCCTATC3500.046.80890333
ACGAGAC4650.046.64332622
GTATGCC4000.046.6324642
GAGCCTA4500.046.1690831
ATCTCGT3500.046.02924337
AGCCTAT4500.045.46880332
CGGGTAT306.905204E-445.16908617
ATCTCCG4900.044.9341210
GTGGTAT3100.044.8153151
Produced by FastQC (version 0.11.5)