FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1494601
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62410.41756963898726146No Hit
GTTTTCTACTGGTCCAACAGTAAGAATTATTTGACTTGGTATCAGCAGAA53980.36116662574158587No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48020.3212897622843823No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45410.30382690764960013No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41500.27766607944193805No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39710.2656896389069725No Hit
TTTCAGTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGCACGAACTGTGGCTGCACCATCTGTCTT38600.25826290762551346No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35370.23665178867135778No Hit
GTACTGAAATGTTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGTTGGTGATGGTGAGCGTGAAAT34350.2298272247910981No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32160.21517448469524642No Hit
GTTTATTACTGTCAGCAACATTTCAGTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGCACGAA32090.21470613227209134No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29930.20025411464330614No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29800.19938431728601813No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29620.1981799824836194No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29560.19777853754948646No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGTTGGTGATGGTGAGCGTGA29140.194968423010556No Hit
CACCATCACCAACCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAACATTTCAGTACTCCGTGGACGTT28610.19142232609238183No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28320.1894820089107394No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27940.18693952432789757No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT27340.18292507498656832No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24830.16613129524200773No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGT24750.1655960353298305No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24500.16392334810427664No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24260.16231756836774497No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23790.15917291638370376No Hit
GTAGAAAACAGTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA23150.1548908370862859No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGCGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGAGTA23130.15475702210824158No Hit
GCCACAGTTCGTGCGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGG23080.15442248466313083No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTCTA23080.15442248466313083No Hit
GTCCCAGACCCGCTGCCACGGAATCGGTCAGGGACCCCGGATGCCCGGGT22610.1512778326790896No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22300.14920370051940285No Hit
GTGCAGCCACAGTTCGTGCGATTTCCACCTTGGTCCCTTGGCCGAACGTC21690.14512234368905147No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21470.1436503789305641No Hit
ATATTATTGTACGAGAGGTCATCCTGAGAGTTCGTCCTTTTTTGACTCCTGGGGCCGGGGAACCCTGGTCACCGT21320.14264676659523176No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTCTACTGGTCCAA21130.14137552430381087No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20650.13816396483074747No Hit
GTCCCTGACCGATTCCGTGGCAGCGGGTCTGGGACAGATTTCACGCTCAC20530.13736107496248162No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG20220.13528694280279485No Hit
GTGATGGTGAGCGTGAAATCTGTCCCAGACCCGCTGCCACGGAATCGGTC19690.1317408458846207No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19540.1307372335492884No Hit
GAGTACTGAAATGTTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGTTGGTGATGGTGAGCGTGAAAT19130.1279940264993801No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTCTGGGCTGAGGTGGCTTTTT19040.1273918590981807No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18910.12652206174089273No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC18680.12498318949338318No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18640.12471555953729456No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGTTGGTGATGGTGAGCGTGAAATCTGTCCCAGACCCGCTGCCA18600.12444792958120594No Hit
TCAGTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGCACGA18300.12244070491054133No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18080.12096874015205396No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17860.11949677539356658No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17580.11762336570094627No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17400.11641903089854751No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17210.11514778860712657No Hit
GTCAGGGACCCCGGATGCCCGGGTAGATGCCCAGTAAATGAGCAACTGAGGAGACTGACCTGGTTTCTGCTGATA17050.11407726878277212No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC16800.11240458155721829No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16450.1100628194414429No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16320.1091930220841549No Hit
GGATCTCTGGTGTCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG16130.10792177979273399No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16050.10738651988055675No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15990.10698507494642383No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTT15780.1055800176769586No Hit
ACTGAGGAGACTGACCTGGTTTCTGCTGATACCAAGTCAAATAATTCTTACTGTTGGACCAGTAGAAAACAGT15760.10544620269891429No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15580.10424186789651552No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15430.10323825556118323No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15330.10256918067096168No Hit
GGTCTGGGACAGATTTCACGCTCACCATCACCAACCTGCAGGCTGAAGAT15230.10190010578074016No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG15120.10116412340149646No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15100.10103030842345215No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15080.10089649344540784No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15070.10082958595638569No Hit
GACCGATTCCGTGGCAGCGGGTCTGGGACAGATTTCACGCTCACCATCAC15050.10069577097834138No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15000.1003612335332306No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)