FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819684
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25850.3153654335085228No Hit
ACATAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGATACATCCAAGAGGGCCACTGGCATCCCTCCCAGGTT24320.29669970378828914No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22000.2683961136242747No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21180.2583922584800972No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19460.23740856232401752No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT19100.2330166259192567No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16350.19946711171622233No Hit
GTTTATTACTGTCAGCAGCGTAGCAACAGGCCTTCGCTCACTTTCGGCGG16090.19629515764611727No Hit
GTCTTTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTTACACCTACTTAGCCTGGTA15670.19117123184056295No Hit
GGTTTATGTTGGTACCAGGCTAAGTAGGTGTAAAGACTCTGACTGGCCCTGCAGGAGAGGGTGGCACTTTCCC14680.1790934067274706No Hit
GTCTTTACACCTACTTAGCCTGGTACCAACATAAACCTGGCCAGGCTCCC14500.17689743852509016No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC14480.176653442058159No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13850.1689675533498275No Hit
GTCTCCGGCCACCCTGTCTTTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTTACAC13750.1677475710151717No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGAAGGC13680.16689358338091265No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCGCAGACCCACTGCCA13420.16372162931080758No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13300.1622576505092206No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13150.16042767700723692No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12860.15688972823673514No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12720.155181752968217No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12520.15274178829890545No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12350.1506678183299906No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12230.14920383952840363No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTTACACCTACTTAGCCTGGTACCAACATAAACCTGGCCAGGCTCCCAGACT12110.14773986072681666No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12020.14664187662562644No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11730.14310392785512466No Hit
TACTTAGCCTGGTACCAACATAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGATACATCCAAGAGGGCCA10520.12834214160578955No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT10120.12346221226716637No Hit
CTCCCAGACTCCTCATCTATGATACATCCAAGAGGGCCACTGGCATCCCTCCCAGGTTCAGTGGCAGTGGGTCTG10100.12321821580023522No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9700.11833828646161204No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCGCAGACCCACTGCCA9650.11772829529428414No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGAAGGCCTGTTGCTACGCTG9540.11638631472616276No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9420.11492233592457582No Hit
GTGTAAAGACTCTGACTGGCCCTGCAGGAGAGGGTGGCACTTTCCCCTGGAGACAAAGACAGGGTGGCCGGAGA9160.11175038185447074No Hit
GTGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTTACACCTACTTAGCCTGGTACCAACATAAACCTGGCCAGG9040.1102864030528838No Hit
GTCAGAGTCTTTACACCTACTTAGCCTGGTACCAACATAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGATA9040.1102864030528838No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8990.1096764118855559No Hit
GTCTTGACACAGTCTCCGGCCACCCTGTCTTTGTCTCCAGGGGAAAGTGC8940.10906642071822802No Hit
GTCTGGGAGCCTGGCCAGGTTTATGTTGGTACCAGGCTAAGTAGGTGTAA8840.1078464383835722No Hit
GCGTAGCAACAGGCCTTCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT8760.10687045251584756No Hit
GTAGTAAATGGCTGTGTCCTCGGATTTCAGGGCATCCATTTGCAGATAGG8550.1043084896130704No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8530.10406449314613925No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8470.10333250374534578No Hit
TTATTACTGTCAGCAGCGTAGCAACAGGCCTTCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA8410.10260051434455231No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8400.10247851611108671No Hit
GAGTGAAGTCTGTCGCAGACCCACTGCCACTGAACCTGGGAGGGATGCCA8210.1001605496752407No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACG200.007810239750.9755673
TCGTATG4400.041.51999740
CGTATGC4400.040.8199541
ATCTCGT3950.040.8097437
ACCGTAC4100.040.67423227
GACCGTA4200.040.49385526
ATGCCGT4300.039.5261344
GGTATCA8800.039.3974231
CCCACGA4700.039.04749719
GTACTAG4400.038.72415530
ACTAGAT4350.038.4399632
CGTACTA4350.038.36944629
CCGTCTT4450.038.35618647
TACTAGA4550.038.2151931
ACGAGAC4650.038.01256622
AGACCGT4500.037.78503425
CTAGATC4000.037.56770733
CGTCTTC4750.037.4683148
ACGGGTC558.197294E-637.0731416
GTATCAA22950.037.026071
Produced by FastQC (version 0.11.5)