FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819684
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41120.5016567360104626No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25150.30682555716593224No Hit
ACATAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGATACATCCAAGAGGGCCACTGGCATCCCTCCCAGGTT21810.2660781471884287No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20570.2509503662386969No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17950.21898682907071504No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16780.20471303575524225No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15880.1937331947433401No Hit
GTCTTTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTTACACCTACTTAGCCTGGTA15860.19348919827640895No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15580.19007324773937273No Hit
GGTTTATGTTGGTACCAGGCTAAGTAGGTGTAAAGACTCTGACTGGCCCTGCAGGAGAGGGTGGCACTTTCCC15420.18812127600392345No Hit
GTTTATTACTGTCAGCAGCGTAGCAACAGGCCTTCGCTCACTTTCGGCGG15230.18580330956807745No Hit
GTCTTTACACCTACTTAGCCTGGTACCAACATAAACCTGGCCAGGCTCCC14640.17860541379360828No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCGCAGACCCACTGCCA13890.1694555462836898No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13430.16384362754427317No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGAAGGC13210.1611596664080304No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC13180.16079367170763367No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12830.1565237335363384No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12760.15566974590207933No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTTACACCTACTTAGCCTGGTACCAACATAAACCTGGCCAGGCTCCCAGACT11750.14334792432205581No Hit
GTCTCCGGCCACCCTGTCTTTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTTACAC11450.13968797731808844No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11360.13858999321689822No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11210.13676001971491453No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10620.12956212394044533No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10360.1263901698703403No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10260.12517018753568449No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10260.12517018753568449No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGAAGGCCTGTTGCTACGCTG10070.12285222109983847No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9540.11638631472616276No Hit
GTGTAAAGACTCTGACTGGCCCTGCAGGAGAGGGTGGCACTTTCCCCTGGAGACAAAGACAGGGTGGCCGGAGA9540.11638631472616276No Hit
GAGTGAAGTCTGTCGCAGACCCACTGCCACTGAACCTGGGAGGGATGCCA9490.11577632355883488No Hit
TACTTAGCCTGGTACCAACATAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGATACATCCAAGAGGGCCA9350.11406834829031676No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9290.11333635888952327No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9210.11236037302179865No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCGCAGACCCACTGCCA9010.10992040835248705No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8960.10931041718515917No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT8910.10870042601783127No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8760.10687045251584756No Hit
TTATTACTGTCAGCAGCGTAGCAACAGGCCTTCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA8740.10662645604891641No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8640.10540647371426062No Hit
GTCTGGGAGCCTGGCCAGGTTTATGTTGGTACCAGGCTAAGTAGGTGTAA8440.10296650904494903No Hit
GTGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTTACACCTACTTAGCCTGGTACCAACATAAACCTGGCCAGG8270.10089253907603418No Hit
CTCCCAGACTCCTCATCTATGATACATCCAAGAGGGCCACTGGCATCCCTCCCAGGTTCAGTGGCAGTGGGTCTG8210.1001605496752407No Hit
GTAGTAAATGGCTGTGTCCTCGGATTTCAGGGCATCCATTTGCAGATAGG8210.1001605496752407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)