FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359314
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATACTTACTCTCCCCGCTACATTTTTGGCCAGGGGACCAAGCTGG91970.676591280601833No Hit
GTATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC87400.6429713811525519No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49310.36275650806215487No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47430.34892600238061255No Hit
GTATTAGTACCTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGTTCTTGATCTATAAGGCGTCTA42420.312069176069694No Hit
GAGTATTAGTACCTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGTTCTTGATCTATAAGGCGT42260.3108921117563712No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37130.2731524872104606No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35610.26197037623389446No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32680.2404153859961716No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC31770.23372083271414848No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31380.23085173845042425No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29680.21834543012137003No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28580.21025311296727617No Hit
CCTTCCACCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCG28510.20973814733019747No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28380.2087817825756227No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27870.20502989007690642No Hit
GTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGAAGCAGACAGGGTGGAAGGAGA27120.19951240110820606No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26570.19546624253115907No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26500.19495127689408037No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26210.19281784782618291No Hit
GAGTAAGTATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTG25580.1881831570924746No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACACTATAATACTTA24850.18281280116293955No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24710.18178286988878214No Hit
ACCTTGAAGGGACCCCACTTTCTAAAGTAGACGCCTTATAGATCAAGAACTTAGGGGCTTTCCCTGGTTTTTG24520.18038510601671137No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA24090.17722174567465648No Hit
CTTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG23700.17435265141093229No Hit
TACTTACTCTCCCCGCTACATTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT23210.17074789195138135No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC23150.17030649283388533No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22470.16530396950226364No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21970.16162564352313005No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAAATGTGACAT21330.15691738626983906No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21040.15478395720194157No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20930.15397472548653218No Hit
ACTTTAGAAAGTGGGGTCCCTTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC20890.1536804594082015No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20870.15353332636903616No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20610.15162059685988669No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20530.1510320647032253No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19990.14705947264576102No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19280.14183624975539133No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18630.13705442598251766No Hit
CCTTATAGATCAAGAACTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACCAGGTACTAATACTCTGA18580.1366865933846043No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18560.13653946034543896No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC18510.1361716277475256No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTACCTGGTTGGCCTGGTATCAGCAAAAA18400.1353623960321162No Hit
GTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA17950.13205190265089597No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17590.12940350794591976No Hit
TTCTAAAGTAGACGCCTTATAGATCAAGAACTTAGGGGCTTTCCCTGGTT17560.12918280838717175No Hit
GTCTCCTTCCACCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAC17160.12624014760386487No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAATG17140.12609301456469954No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16830.12381245245763671No Hit
ACTTATTACTGCCAACACTATAATACTTACTCTCCCCGCTACATTTTTGGCCAGGGGACCAAGCTGGAGATCAAA16670.12263538814431398No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16410.1207226586351645No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16270.1196927273610071No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16210.11925132824351108No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15940.11726503221477892No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGAAGGGACCCCA15820.11638223397978685No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15810.1163086674602042No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15730.11572013530354282No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15710.11557300226437747No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15320.11270390800065327No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15300.11255677496148793No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14990.1102762128544251No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14930.10983481373692908No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14730.10836348334527562No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14670.1079220842277796No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14490.10659788687529151No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG14140.104023058689898No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14040.10328739349407126No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13950.10262529481782723No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13950.10262529481782723No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13830.10174249658283516No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13690.10071256530867775No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13680.10063899878909507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT400.006611413353.16855270
GCGACGC200.007573343351.37608737
CTCGCGA200.00772826351.11361732
CGAAACC752.2088534E-741.6892569
CGTATGC3050.038.42276441
TCGTATG3050.038.3509640
GTGGTAT2450.035.9451071
GTATCAA23200.035.623321
GTTATTA1001.5871011E-435.445770
TGCACCA39800.034.73322770
GTATGCC3350.034.01800542
TATACAC4300.033.8588943
ATCTCGT2950.033.67020837
ACGAGAC4050.033.46426822
AATCTCG3150.032.58215336
CTTATAC4600.032.3986851
CGAGACA4200.032.27268223
ATGCCGT3350.032.0597544
TTATACA4450.031.9567072
GTCGTAT652.3954593E-531.70098739
Produced by FastQC (version 0.11.5)