FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359314
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC88660.6522407626199686No Hit
CTATAATACTTACTCTCCCCGCTACATTTTTGGCCAGGGGACCAAGCTGG82850.6094986147424363No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49090.3611380446313361No Hit
GAGTATTAGTACCTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGTTCTTGATCTATAAGGCGT40740.29971000077980514No Hit
GTATTAGTACCTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGTTCTTGATCTATAAGGCGTCTA39210.28845432328365633No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38660.28440816470660935No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37940.27911137529665697No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36860.2711661911817284No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34140.2511560978552417No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30460.22408361864881846No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC29320.21569703541639385No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28740.21143017728059887No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28540.20995884688894545No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27850.20488275703774111No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27470.20208722929359957No Hit
GTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGAAGCAGACAGGGTGGAAGGAGA26370.19399491213950565No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25680.1889188222883013No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25510.1876681914553959No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACACTATAATACTTA25360.18656469366165582No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25290.18604972802457712No Hit
GAGTAAGTATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTG24010.1766332135179951No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG23660.1740583853326016No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23240.17096859151012936No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23180.17052719239263334No Hit
CCTTCCACCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCG23060.16964439415764127No Hit
TACTTACTCTCCCCGCTACATTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT22560.16596606817850768No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA21970.16162564352313005No Hit
ACCTTGAAGGGACCCCACTTTCTAAAGTAGACGCCTTATAGATCAAGAACTTAGGGGCTTTCCCTGGTTTTTG21830.16059571224897262No Hit
CTTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG21750.16000718009231127No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21700.1596393474943979No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC21670.1594186479356499No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGAAGGGACCCCA20810.15309192725154014No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20740.1525769616144614No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20680.1521355624969654No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20640.1518412964186347No Hit
ACTTTAGAAAGTGGGGTCCCTTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC20150.1482365369590838No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAAATGTGACAT19680.1447789105386982No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19590.14411681186245415No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19340.14227764887288735No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19070.14029135284415523No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18490.13602449470836026No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17990.13234616872922664No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC17780.13080127181799053No Hit
TTCTAAAGTAGACGCCTTATAGATCAAGAACTTAGGGGCTTTCCCTGGTT17760.1306541387788252No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17570.12925637490675443No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17520.12888854230884106No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17440.1283000101521797No Hit
GTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA17310.12734364539760495No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17140.12609301456469954No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17040.12535734936887283No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTACCTGGTTGGCCTGGTATCAGCAAAAA16940.1246216841730461No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAATG16930.12454811765346345No Hit
CCTTATAGATCAAGAACTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACCAGGTACTAATACTCTGA16850.12395958549680207No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16570.12189972294848726No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16550.1217525899093219No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16050.11807426393018831No Hit
ACTTATTACTGCCAACACTATAATACTTACTCTCCCCGCTACATTTTTGGCCAGGGGACCAAGCTGGAGATCAAA15910.11704433265603091No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15780.11608796790145617No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15580.11461663750980274No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15450.11366027275522801No Hit
GTCTCCTTCCACCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAC15200.1118211097656612No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15050.11071761197192113No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15020.11049691241317311No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGTACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG14850.1092462815802677No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG14700.10814278378652761No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14570.10718641903195289No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14440.10623005427737814No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14060.10343452653323662No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13940.10255172829824455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)