FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634564
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC63930.39111347123759No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAACACCCG61650.3771647974628096No Hit
GTAGTATACAGCCGTGTCCTCGTCTCTCAGGCTGAGCATTTGCAGAGACA61320.3751459104690914No Hit
GGATGATAGTGTGAATGGTCGGGTGTTCGGCGGAGGGACGAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC55320.3384388742196696No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGACACCCTCAGCGTCTGGGACCC49340.3018541947577458No Hit
GACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAACACCCGACCATTCACA47640.2914538678204096No Hit
GTATACTACTGTGTGAGAGACAAAGCAGCTCGTTTCGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGT46780.2861925259579925No Hit
CTATCATCCCATGCTTCACAGTAATAATCACCCTCATCCTCAGACTGGAG45140.2761592693831505No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44260.27077557073323527No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG40500.24777249468359758No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40170.2457536076898794No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGACACCCTCAGCGTCT38240.23394617769631537No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTC37290.2281342302901569No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA34740.21253373988415258No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC34180.20910774983420657No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC33850.20708886284048836No Hit
GATTATTACTGTGAAGCATGGGATGATAGTGTGAATGGTCGGGTGTTCGG33750.206477078902998No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG31310.19154955082823308No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT31120.1903871613470014No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG30520.18671645772205922No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG29650.18139393746589305No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28850.1764996659659701No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28680.17545963327223651No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28420.17386899503476155No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA27010.16524284151614743No Hit
GTAATAATCACCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG26840.1642028088224138No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG26780.16383573845991958No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26580.16261217058493885No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26350.16120506752871103No Hit
ACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAACACCCGACCATTCACAC25670.15704493675377654No Hit
CCCCTGAGGGCCGCTGATTATTAGTATAGATGAGGAGTTTGGGGGCCGTTCGTGGGAGCTGCTGGTACCAGTTTA25540.15624961763503906No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGACAC24920.1524565572225988No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT24530.15007059986638638No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24130.14762346411642494No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC23190.1418726951040155No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23120.14144444634777226No Hit
GTCTGAGGATGAGGGTGATTATTACTGTGAAGCATGGGATGATAGTGTGA23070.14113855437902706No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22750.1391808457790579No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22340.1366725316353474No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21940.13422539588538596No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATAGC21760.13312418479790328No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21330.13049351386669472No Hit
GTGCTGACTCAGACACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC21230.12988172992920438No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21110.12914758920421593No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20990.12841344847922748No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA20830.1274345941792429No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20770.12706752381674868No Hit
CATCTATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTCAGCCTC20660.1263945614855093No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGGTGATTATTACTGTGAAG20550.12572159915426986No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19820.12125557641059022No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19700.12052143568560178No Hit
GCTGTATACTACTGTGTGAGAGACAAAGCAGCTCGTTTCGCTTTTGATAT19580.11978729496061336No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAACAC19530.11948140299186817No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG19390.11862490547938165No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGGCAGAGAATCGGTCAGGGACC19190.11740133760440093No Hit
GTGTAAACTGGTACCAGCAGCTCCCACGAACGGCCCCCAAACTCCTCATC19130.1170342672419067No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG19130.1170342672419067No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19030.11642248330441636No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC18800.11501538024818851No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18540.11342474201071356No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT18140.11097760626075209No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18100.11073289268575595No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17880.10938696802327715No Hit
CTATTACTTCCGATGTCGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT17770.10871400569203776No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTATTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT17680.10816340014829642No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17680.10816340014829642No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17550.10736808102955896No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17510.10712336745456282No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC17400.1064504051233234No Hit
GTATAGATGAGGAGTTTGGGGGCCGTTCGTGGGAGCTGCTGGTACCAGTTTACACTATTACTTCCGATGTCGGAG17180.1051044804608446No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG17130.10479858849209943No Hit
GATTATTAGTATAGATGAGGAGTTTGGGGGCCGTTCGTGGGAGCTGCTGG17020.10412562616086002No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGGCAGAGAATCGGTC16780.10265734471088316No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16780.10265734471088316No Hit
TTATTACTGTGAAGCATGGGATGATAGTGTGAATGGTCGGGTGTTCGGCG16660.1019232039858947No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC16590.10149495522965145No Hit
CTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAG16420.10045492253591784No Hit
CAGTAGTATACAGCCGTGTCCTCGTCTCTCAGGCTGAGCATTTGCAGAGA16400.10033256574841976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4400.057.79105470
TTATCGT200.00735240751.76273741
GTCGGTA200.007801914650.99160412
TCTGGAG21000.046.41630670
GGTATCA9450.045.3425181
CGCGTAT851.8189894E-1244.5391139
ATACGTA551.651133E-743.26560211
GCGTATG803.45608E-1143.0647440
AATCGCG903.6379788E-1241.91671836
TACGTAC603.2690878E-739.66013712
GTATCAA24200.039.2062841
CGTATGC3900.037.16299441
CTCTTAT2750.037.0984231
ACGAGAC6750.036.76881822
TTATACA6850.036.22652
TATACAC7100.035.908483
CACGAGA6850.035.73352421
CTTATAC6950.035.7194631
CGCTCAT6650.034.83365228
TCTCCGA7150.034.70757311
Produced by FastQC (version 0.11.5)