FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004689598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634564
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC63570.38891104906262464No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60290.3688445359129407No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGACACCCTCAGCGTCTGGGACCC58130.35563000286314883No Hit
GTAGTATACAGCCGTGTCCTCGTCTCTCAGGCTGAGCATTTGCAGAGACA55420.33905065815715996No Hit
GGATGATAGTGTGAATGGTCGGGTGTTCGGCGGAGGGACGAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC55100.33709294955719077No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAACACCCG49900.30528018480769187No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48600.2973269936203171No Hit
GTATACTACTGTGTGAGAGACAAAGCAGCTCGTTTCGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGT45120.27603691259565244No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGACACCCTCAGCGTCT44220.2705308571582391No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG38150.233395572152574No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37520.22954133334638474No Hit
GACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAACACCCGACCATTCACA35300.21595972993409865No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35010.21418555651537657No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT31800.1945472921219359No Hit
GATTATTACTGTGAAGCATGGGATGATAGTGTGAATGGTCGGGTGTTCGG31770.19436375694068878No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG31700.1939355081844455No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTC31570.19314018906570804No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31510.19277311870321384No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31500.19271194030946479No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC31080.19014244777200526No Hit
CTATCATCCCATGCTTCACAGTAATAATCACCCTCATCCTCAGACTGGAG30140.18439167875959583No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG29410.17992565601591617No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28460.1741137086097577No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT27710.16952532907857998No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27180.16628287420988105No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT27080.1656710902723907No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGACAC26530.16230627861619368No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG26410.16157213789120525No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26240.16053210519747163No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAACAC24620.15062120541012772No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24390.1492141023538999No Hit
CCCCTGAGGGCCGCTGATTATTAGTATAGATGAGGAGTTTGGGGGCCGTTCGTGGGAGCTGCTGGTACCAGTTTA24160.14780699929767205No Hit
GTCTGAGGATGAGGGTGATTATTACTGTGAAGCATGGGATGATAGTGTGA24100.14743992893517782No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23740.14523750676021252No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23150.14162798152901934No Hit
GTGCTGACTCAGACACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC23060.14107737598527803No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA22940.14034323526028958No Hit
CATCTATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTCAGCCTC22810.13954791614155213No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG22670.1386914186290656No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22530.1378349211165791No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21160.1294534811729611No Hit
GTGTAAACTGGTACCAGCAGCTCCCACGAACGGCCCCCAAACTCCTCATC20740.12688398863550157No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC20650.12633338309176026No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATAGC20410.12486510164178338No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT20410.12486510164178338No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20390.1247427448542853No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20330.1243756744917911No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20110.1230297498293123No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20100.12296857143556325No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20090.12290739304181421No Hit
ACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAACACCCGACCATTCACAC20000.12235678749807288No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGGTGATTATTACTGTGAAG19960.12211207392307673No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG19740.12076614926059794No Hit
CTCTTGTTCTGGAAGCAGCTCCGACATCGGAAGTAATAGTGTAAACTGGTACCAGCAGCTCCCACGAACGGCCCC19380.11856372708563263No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19190.11740133760440093No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC18850.1153212722169337No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18710.11446477470444719No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18700.11440359631069814No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18660.11415888273570199No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18350.11226235252948186No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTATTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT18140.11097760626075209No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18130.11091642786700305No Hit
CTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAG18050.11042700071701077No Hit
GTTTGGGGGCCGTTCGTGGGAGCTGCTGGTACCAGTTTACACTATTACTT18000.11012110874826558No Hit
GCTGTATACTACTGTGTGAGAGACAAAGCAGCTCGTTTCGCTTTTGATAT17930.10969285999202233No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC17850.10920343284203005No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17840.109142254448281No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17680.10816340014829642No Hit
TTATTACTGTGAAGCATGGGATGATAGTGTGAATGGTCGGGTGTTCGGCG17670.1081022217545474No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17610.10773515139205316No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA17440.10669511869831955No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17440.10669511869831955No Hit
ATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTCAGCCTCCCTGG17380.10632804833582533No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC17310.10589979957958207No Hit
CTATTACTTCCGATGTCGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT17260.10559390761083691No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG17250.10553272921708787No Hit
GTATAGATGAGGAGTTTGGGGGCCGTTCGTGGGAGCTGCTGGTACCAGTTTACACTATTACTTCCGATGTCGGAG17150.1049209452795975No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT17010.10406444776711098No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16840.10302441507337735No Hit
GTGCTGGGTTTTCCTTATTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC16500.10094434968591012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)