FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082720
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63790.5891643268804493No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30590.28252918575439634No Hit
CTTTAGGAGGCTGTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGTTGAATAAAACACT26950.24891015220925078No Hit
ATATTATATTTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT26920.24863307226245013No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25420.2347790749224176No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23820.22000147775971626No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA22190.20494680065021426No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20900.19303236293778633No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18630.17206664696320378No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18510.17095832717600118No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18330.16929584749519727No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17230.15913624944584012No Hit
GTTTATTACTGTCAACAATATTATATTTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA17220.15904388946357323No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17160.15848972956997193No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17150.15839736958770503No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16400.15147037091768878No Hit
CTCCTAAAGTGATCCTTTACTGGGCATCTACCCGGGAATTTGGGGTCCCT15900.1468523718043446No Hit
ATATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC15550.14361977242500368No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15330.14158785281513225No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15070.13918649327619328No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13250.12237697650362052No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13100.12099157676961726No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC12930.11942145707108025No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGCTCAGGGACCCCA12780.118036057337077No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12560.11600413772720555No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGTTTCAGTTTCACTAATGCCTGG12200.11267917836559777No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATATTTCC11910.11000073887985813No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11750.108522979163588No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11600.10713757942958475No Hit
GTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGTTG11490.10612161962464903No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11130.10279666026304123No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11080.10233486035170683No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11030.10187306044037239No Hit
CTCCTGTGCAGCCTCTGGTTTCAGTTTCACTAATGCCTGGATGAACTGGGTTCGCCAGGCTCCAGGGAAGGGG10980.10141126052903798No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10870.10039530072410226No Hit
GTCCCAGACCCGCTGCCACTGAATCGCTCAGGGACCCCAAATTCCCGGGT10860.10030294074183538No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAA451.13893926E-465.0541370
CCGTTTA406.4051425E-445.8608869
GGTATCA17800.045.0149541
TGGTATC4350.041.622062
GTATCAA49200.041.157671
TATCAAC55650.036.0292362
ATCAACG57600.034.8628773
TCAACGC57800.034.394244
GTGGTAT5700.034.1139561
CAACGCA59100.033.6959655
AACGCAG59750.033.5553866
ACGCAGA63250.031.5395457
TGTTAGA12150.031.3223670
CGCAGAG64700.030.936358
CACCGTT500.00473471630.51637867
AGAGTAC66750.030.43548611
GCAGAGT66550.030.3282579
CAGAGTA67300.030.13972910
TGCACAG6000.029.27435970
TTGGTAG954.81435E-628.1071967
Produced by FastQC (version 0.11.5)