FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073056
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATATAGTTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAAAA105290.9812162645751946No Hit
ATATAAGACAGTCTGACTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA48800.45477589240449706No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41180.38376375510690963No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35480.3306444398055647No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29190.2720268094116243No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27340.2547863298839949No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG25540.23801180926251753No Hit
GTTTATTACTGTCAGCAATATTTTGCTATTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA23920.2229147407031879No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT23170.21592535711090566No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21540.20073509677034562No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21430.1997099871768109No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20660.19253422002206783No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20480.19085676795992007No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20290.1890861241165419No Hit
ACTTAGGGGGCTGTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAACTATATAAGACAGT20030.18666313780455074No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19850.184985685742403No Hit
GTACAAGGCTGTGTCCTCGATTTTCAGGCTGTTCATTTGCAGATACAACG19760.18414695971132913No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC19650.18312185011779442No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19240.1793009870873468No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19130.17827587749381205No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18950.17659842543166432No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTTTGCTATT18480.17221841171383415No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17960.16737243908985178No Hit
CCCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT17900.1668132884024692No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17370.16187412399725642No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17110.15945113768526525No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA16860.15712134315450452No Hit
AACCAGGACAGCCCCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAA16690.15553708287358722No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16630.15497793218620465No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC15970.1488272746249963No Hit
GCCTTGTACTTCTGCACGACCGGGGGAGTGGTTTGGGGAGTTAAAGTCTA15960.14873408284376585No Hit
GTCCCTGACCGATTCACTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC15480.14426087734470522No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15220.14183789103271405No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGATGGATTTTC15090.14062639787671846No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14400.1341961649718188No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14100.13140041153490592No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGACTGTCTTATATAGTTCCAC13640.12711358959830615No Hit
GTCTTATATAGTTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCCCCTAAGTTGCT13600.12674082247338442No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13190.12291995944293682No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13160.12264038409924552No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGT12640.11779441147526316No Hit
CTACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCCCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATC12530.11676930188172846No Hit
TTGCTATTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA12400.11555780872573285No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12360.11518504160081115No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12330.11490546625711985No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12230.11397354844481557No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12170.11341439775743299No Hit
GTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAACTA12100.11276205528881995No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTTTGCTATTCCGTGGACGTT12080.11257567172635911No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11960.11145737035159395No Hit
GGCAGGGGCAGCAAGATGGTGTTGCGGACCCAGGTCTTCATTTCTCTGTT11890.11080502788298095No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11830.11024587719559836No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGGGGCTGTCCTGGTTTTTGCTGGTA11730.10931395938329407No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11670.10875480869591149No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11580.10791608266483763No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGATGGA11570.1078228908836072No Hit
ACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCCCCTAAGTTGCTCATT11550.10763650732114632No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11050.10297691825962485No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT10930.1018586168848597No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10890.10148584975993796No Hit
GCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA10860.10120627441624669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT4350.048.92722737
CTTATAC5350.048.925291
ATGCCGT5500.048.59211744
TCGTATG5400.048.54194340
CGTATGC5500.048.348241
GCCGTCT5500.048.0892246
GTATGCC5600.047.57044242
TGCCGTC5650.047.36292345
CACGAGA5600.047.3569821
GACAGGT1300.047.0679861
ACATGCG5500.047.04306827
ACGAGAC5650.046.94008322
GGTATCA7150.046.1171151
ATCGTAT451.8843821E-645.9176139
GCGCAGA5600.045.7124531
TATACAC5850.045.3078273
CCGTCTT5850.045.28339847
CATGCGC5750.045.0292528
CGAGACA5900.044.95947623
GATCTCG4750.044.76700236
Produced by FastQC (version 0.11.5)