FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659295
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCACCGTGGGTTAATAGCCTAAATGGTCCGATGTTCGC34520.5235895919125733No Hit
GTAATAATCAGCCTCATCCTCAGGCTGGAGCCCACTGATGGCCAGGGAGG29010.4400154710713717No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC24930.37813118558460174No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGGCGAACATCGG19120.29000674963407885No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17440.26452498502187943No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16650.2525424885673333No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGGCGAACATCGGACCATTTAGG15870.24071166928309787No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14440.21902183392866625No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14320.21720170788493773No Hit
GTCTTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCTGGGACTC13950.21158965258344142No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12980.1968769670633025No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12700.19263000629460258No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12550.1903548487399419No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11920.18079918701036712No Hit
GTAATAGACGGCCGTGTCCTCGGCTCGGAGGCTGTTCATCTGCAGATACA11780.17867570662601717No Hit
GTGCAGGGTCTTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCT11430.1733670056651423No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11090.16820998187457814No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10980.16654153300116034No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT10940.1659348243199175No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10890.16517643846836394No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10820.16411469827618896No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10800.16381134393556754No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC10750.163052958084014No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10470.15880599731531408No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10140.15380065069506063No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10060.15258723333257496No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9480.14378995745455372No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9470.14363828028424303No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9350.14181815424051447No Hit
TTATTATAGATGAGGAGTTTGGGGGCCGCTCCTGGGAACTGCTGGTACCA9330.14151479989989307No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9300.14105976838896095No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGGCGAACATCGGACCATTTAGGC9090.13787454781243602No Hit
CCCCTGAGGGCCTCTGATCATTATTATAGATGAGGAGTTTGGGGGCCGCTCCTGGGAACTGCTGGTACCAGTTAA9090.13787454781243602No Hit
TTATTACTGTGCACCGTGGGTTAATAGCCTAAATGGTCCGATGTTCGCCG9040.13711616196088247No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8990.1363577761093289No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG8840.13408261855466824No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8770.13302087836249327No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8680.1316557838296969No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8680.1316557838296969No Hit
ACTGTGCACCGTGGGTTAATAGCCTAAATGGTCCGATGTTCGCCGGAGGGACCAGGCTGACCGTCCTAGGTCAGC8640.13104907514845401No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG8590.13029068929690046No Hit
CCTCACTCACTGTGCAGGGTCTTGGGCCCAGTCTGCGCTGACTCAGCCAC8310.1260437285282006No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8060.12225179927043281No Hit
CTCCTGGGAACTGCTGGTACCAGTTAACAGTTTGACTTCCGATGTTGGAGTAGCTTCCAGAACAAGAGATGGTGA8040.1219484449298114No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGGTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG7820.11861154718297576No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA7760.1177014841611115No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7750.11754980699080078No Hit
CTTCAGGTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC7710.11694309830955793No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGC7520.11406123207365444No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA7380.11193775168930448No Hit
GATATATCCTATGTGGACTCGGCAAGGGGCCGATTCACCATCTCCAGAGACAACGCCGAGAATTCACTGTATCTG7350.11148272017837235No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC7290.11057265715650809No Hit
CAGTAATAATCAGCCTCATCCTCAGGCTGGAGCCCACTGATGGCCAGGGA7250.10996594847526525No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGCCTGAGGATG7150.10844917677215814No Hit
GCCTGGTCCCTCCGGCGAACATCGGACCATTTAGGCTATTAACCCACGGTGCACAGTAATAATCAGCCTCATCCT7110.1078424680909153No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC6940.10526395619563321No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG6930.10511227902532251No Hit
CTATAATAATGATCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC6890.10450557034407965No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCTTGGGCCCAGT6740.102230412789419No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6730.1020787356191083No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC6710.10177538127848687No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC6690.10147202693786546No Hit
CCCTCAGCGTCTGGGACTCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG6640.1007136410863119No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT6630.10056196391600117No Hit
GGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG6600.10010693240506904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)