FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004689604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659295
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCACCGTGGGTTAATAGCCTAAATGGTCCGATGTTCGC33660.5105453552658522No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27870.42272427365595067No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC21830.3311112627882814No Hit
GTCTTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCTGGGACTC18250.27681083581704696No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17420.26422163068125804No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15300.23206607057538733No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGGCGAACATCGG14260.21629164486307345No Hit
GTGCAGGGTCTTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCT13700.20779772332567364No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13580.20597759728194512No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12820.19445013233833108No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12530.19005149439932048No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12420.18838304552590265No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12330.18701795099310628No Hit
GTAATAATCAGCCTCATCCTCAGGCTGGAGCCCACTGATGGCCAGGGAGG12230.18550117928999918No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11340.17200191113234592No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11030.1672999188527139No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC10970.16638985583084961No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10920.16563146997929606No Hit
GTAATAGACGGCCGTGTCCTCGGCTCGGAGGCTGTTCATCTGCAGATACA10820.16411469827618896No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGGCGAACATCGGACCATTTAGG10760.16320463525432471No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10370.15728922561220698No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG10330.15668251693096413No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10170.15425568220599276No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9890.15000872143729288No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG9860.14955368992636073No Hit
CCTCACTCACTGTGCAGGGTCTTGGGCCCAGTCTGCGCTGACTCAGCCAC9770.14818859539356435No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9670.14667182369045723No Hit
TTATTATAGATGAGGAGTTTGGGGGCCGCTCCTGGGAACTGCTGGTACCA9350.14181815424051447No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9200.13954299668585382No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8950.13575106742808607No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC8930.13544771308746464No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8850.13423429572497894No Hit
TTATTACTGTGCACCGTGGGTTAATAGCCTAAATGGTCCGATGTTCGCCG8760.13286920119218254No Hit
ACTGTGCACCGTGGGTTAATAGCCTAAATGGTCCGATGTTCGCCGGAGGGACCAGGCTGACCGTCCTAGGTCAGC8750.13271752402187184No Hit
GGTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA8700.1319591381703183No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8650.13120075231876474No Hit
CCCCTGAGGGCCTCTGATCATTATTATAGATGAGGAGTTTGGGGGCCGCTCCTGGGAACTGCTGGTACCAGTTAA8590.13029068929690046No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8560.12983565778596834No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGC8200.12437527965478276No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGGTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG8180.12407192531416134No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA7990.12119005907825783No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7960.12073502756732571No Hit
CTTCAGGTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC7950.120583350397015No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA7910.11997664171577214No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7910.11997664171577214No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC7840.11891490152359718No Hit
CTATAATAATGATCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC7830.11876322435328646No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCTTGGGCCCAGT7750.11754980699080078No Hit
CTCCTGGGAACTGCTGGTACCAGTTAACAGTTTGACTTCCGATGTTGGAGTAGCTTCCAGAACAAGAGATGGTGA7730.11724645265017936No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7590.1151229722658294No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG7580.1149712950955187No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGCCTGAGGATG7540.11436458641427587No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7530.11421290924396514No Hit
CTCTTGTTCTGGAAGCTACTCCAACATCGGAAGTCAAACTGTTAACTGGTACCAGCAGTTCCCAGGAGCGGCCCC7500.113757877733033No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG7490.1136062005627223No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC7300.11072433432681879No Hit
CCCTCAGCGTCTGGGACTCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG7290.11057265715650809No Hit
GGCCATCAGTGGGCTCCAGCCTGAGGATGAGGCTGATTATTACTGTGCAC7080.10738743657998316No Hit
CTGTTAACTGGTACCAGCAGTTCCCAGGAGCGGCCCCCAAACTCCTCATC7070.10723575940967246No Hit
GAGTACGGGAGCTTCAGGTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC7040.10678072789874034No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6950.10541563336594392No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6910.10480892468470109No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGGCGAACATCGGACCATTTAGGC6770.10268544430035112No Hit
GGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG6750.10238208995972972No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)