FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841115
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGACCTTCCGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAG70390.8368653513491021No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27340.3250447322898771No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT26270.3123235229427605No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25810.30685459182157016No Hit
GGTCTGTACTGTGCATGCAATAATAAACCCCAAGATCCTCAGCCTCCACC20960.24919303543510696No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18920.2249395148106977No Hit
CAATAATACAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCAGTTGCAGATA17090.2031826801329188No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTGCATAGTGATGGAAAGACCTATTTGT16070.19105591982071418No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15670.18630032754141823No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15350.1824958537179815No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15100.17952360854342153No Hit
GTGATGGAAAGACCTATTTGTCTTGGTATCTGCAGAAGCCAGGCCAGCCTCCACAACTCCTGATGTATGAAGTTT14650.17417356722921362No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14550.17298466915938962No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14520.17262799973844242No Hit
CTTTTGGCCAGGGGACCAAACTGGAGATCAAGCGAACTGTGGCTGCACCA13930.1656135011264809No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13550.16109568846114977No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13340.15859900251451942No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13190.15681565540978346No Hit
GTATTATTGTGTGAGAATGGATCCCTGGGCCGCTTTTAACATGTGGGGCC12920.15360563062125868No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12280.1459966829743852No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11830.14064664166017726No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11780.14005219262526528No Hit
GTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGGGACAAATT11730.1394577435903533No Hit
GCACAGTACAGACCTTCCGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAGCGAACTGTGGCTGCACCAT10700.12721209347116624No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCGCTGCAGATATTGTGATGA10510.12495318713850068No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10500.12483429733151827No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10490.12471540752453589No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10030.11924647640334556No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9600.11413421470310244No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9230.1097352918447537No Hit
GGATACCTGGATCCGCTGCAGATATTGTGATGACCCAGACTCCGGTCTCTCTGTCCGTCACCCCTGGACAGCCGG8970.10664415686321134No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC8750.10402858110959856No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8640.10272079323279219No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8620.1024830136188274No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8540.1015318951629682No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTGTACGGAAGGT8490.1009374461280562No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG150.002549076767.637922
GTCGAAC200.006464978753.47163456
CTGTCTT23100.052.2267570
CTAACGC200.00796179950.7284363
CCGTCGT305.9260294E-446.590347
ATCTCGT5650.044.92952737
GTGGTAT2800.044.7105981
TCGTATG5800.044.6064640
CGTCTTC6050.044.5628848
ATGCCGT5950.044.4307844
CGTATGC6150.043.82500541
CCGTCTT6250.043.6085247
ACGAGAC7600.043.1944822
TATACAC7750.042.328243
CCCACGA7600.042.28626619
CGAGCCC7600.041.84363615
CTTATAC7700.041.7445371
CGAGACT7800.041.6654423
GCTAATC6500.041.4653933
TCTCCGA7750.041.46535511
Produced by FastQC (version 0.11.5)