FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841115
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGACCTTCCGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAG65420.77777711727885No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64310.7645803487038039No Hit
GGTCTGTACTGTGCATGCAATAATAAACCCCAAGATCCTCAGCCTCCACC34090.405295352002996No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28740.34168930526741287No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21030.25002526408398373No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18580.22089726137329616No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16990.20199378206309482No Hit
CAATAATACAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCAGTTGCAGATA16150.19200703827657337No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14290.16989353417784728No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14250.16941797494991767No Hit
CTTTTGGCCAGGGGACCAAACTGGAGATCAAGCGAACTGTGGCTGCACCA14200.16882352591500568No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13780.16383015402174494No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13420.1595501209703786No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13180.15669676560280105No Hit
GTATTATTGTGTGAGAATGGATCCCTGGGCCGCTTTTAACATGTGGGGCC12760.1517033937095403No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12280.1459966829743852No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12270.1458777931674028No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12200.14504556451852602No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTGCATAGTGATGGAAAGACCTATTTGT11540.13719883725768772No Hit
GTGATGGAAAGACCTATTTGTCTTGGTATCTGCAGAAGCCAGGCCAGCCTCCACAACTCCTGATGTATGAAGTTT11400.13553437995993414No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11320.13458326150407493No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10990.1306598978736558No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10400.12364539926169431No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10390.1235265094547119No Hit
GTACTGTGCATGCAATAATAAACCCCAAGATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAATTTGTCC9800.1165120108427504No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9710.1154420025799088No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9390.11163752875647207No Hit
GCACAGTACAGACCTTCCGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAGCGAACTGTGGCTGCACCAT9390.11163752875647207No Hit
GTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGGGACAAATT9350.11116196952854246No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9190.10925973261682409No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9130.10854639377492971No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTGTACGGAAGGT9110.10830861416096492No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8540.1015318951629682No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT502.2568225E-456.6495470
GACGAAT200.007472774551.54833238
CTGTCTT21550.047.97466770
GTATCAA32150.039.9890441
GGTATCA11800.039.4633641
CGACTAA350.00139843539.13266436
ACTACCG350.001502639338.5646138
AATTTGT12750.036.10019770
TATCAAC37250.034.33492
TTGACGG601.4503612E-534.11134733
CGTATGG400.002895483733.7400213
ATCAACG37850.033.7054753
TCAACGC38200.033.5693634
AACGCAG38450.033.261356
CAACGCA38900.032.9613655
CGCAGAG39400.032.373728
GTGGTAT3450.032.2769051
AGAGTAC40200.032.05760211
ACGCAGA40100.031.892747
CAGAGTA40750.031.54776210
Produced by FastQC (version 0.11.5)