FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427936
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTGCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT35950.8400788903013535No Hit
GTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGCCTGCTGATGGTGAGAGTGAAATCTGTC25100.58653630449413No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15250.35636169894563674No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT14650.3423409107903986No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14400.336498915725716No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9470.22129477305017575No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8670.2026003888431915No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8520.19909519180438198No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8350.19512263516039782No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8000.18694384206984221No Hit
AGCCAGGACAGCCTCCCAAGTTGCTCATTTACTGGGCATCTACCCGGGAA7510.1754935317430644No Hit
GTTTATTACTGTCAACAATATTATACTGCTCGGACGTTCGGCCAAGGGAC7380.17245569430942945No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7270.1698852164809691No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7190.16801577806027068No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7110.16614633963957226No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7030.16427690121887387No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6570.15352763029985791No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6400.14955507365587378No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5670.13249644806700067No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5660.13226276826441338No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5470.12782285201525462No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5390.1259534135945562No Hit
CCTTTTATACAACTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAAGCCAGGACAGCCTCCCAAGTTGCT5170.12081245793763554No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5150.12034509833246093No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAACCTTTTATA5010.1170735810962387No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC4990.11660622149106407No Hit
CTTTTATACAACTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA4940.11543782247812757No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT4930.11520414267554026No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG4920.11497046287295297No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT4910.11473678307036565No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA4900.11450310326777835No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4840.11310102445225455No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG4830.11286734464966725No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT4650.1086611082030958No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC4570.10679166978239736No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4420.10328647274358783No Hit
GTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGTTG4380.10235175353323861No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAACCTTTTATACAACTCCAA4370.10211807373065132No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATT100.007322923143.4180570
AGTCGGT150.00260976767.2319326
GTACTAG3450.049.93885830
TCGTATG3400.049.6683240
ATCTCGT3050.049.29196537
CTAGATC3100.049.23568733
CGTATGC3450.049.03682341
ACCGTAC3450.048.77020627
ATGCCGT3600.048.35994744
CGTACTA3550.047.51339729
TCTCCGA3600.047.5057611
GACCGTA3550.047.34642826
ACGAGAC3550.047.26338622
ACTAGAT3600.047.0692832
GAGACCG3500.046.99629224
TACTAGA3600.046.96943731
GTATGCC3550.046.8011842
GCCGTCT3600.046.60650346
CGTCTTC3400.046.5073848
GATCTCG3300.046.4934936
Produced by FastQC (version 0.11.5)