FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427936
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTGCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT35920.8393778508935915No Hit
GTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGCCTGCTGATGGTGAGAGTGAAATCTGTC33260.7772190234053691No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29590.691458535855829No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14420.3369662753308906No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10730.2507384281761759No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9660.2257346892993345No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8980.20984446272339788No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7150.1670810588499215No Hit
GTTTATTACTGTCAACAATATTATACTGCTCGGACGTTCGGCCAAGGGAC6900.16123906378523892No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6850.1600706647723024No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6790.15866858595677857No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6780.15843490615419126No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6700.15656546773349286No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6420.15002243326104836No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGCCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA6330.14791931503776265No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6330.14791931503776265No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6280.14675091602482615No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5920.13833844313168323No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5900.13787108352650862No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5620.13132804905406414No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5390.1259534135945562No Hit
AGCCAGGACAGCCTCCCAAGTTGCTCATTTACTGGGCATCTACCCGGGAA5270.12314925596350858No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4980.11637254168847679No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT4850.11333470425484184No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4720.11029686682120692No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG4660.10889478800568309No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT4500.10515591116428626No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4480.10468855155911165No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA4390.10258543333582593No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCA300.00177603874.0678670
CAACGTC200.002424958468.4847569
CGATTGT200.005671787555.26514462
ATCTGGT552.8904394E-453.8675470
GTGGTAT1500.046.8949241
TCGGAGC358.7993004E-442.9913766
GGTATCA5050.041.1242641
AGTACTA350.001145313440.74950853
GATATTA451.1920575E-437.2094964
TGGTATC1850.036.19962
GTATCAA12950.035.9536361
CGGGAAC853.932655E-935.48334517
CTGTCTT11900.035.2974669
GAGTTTA1350.034.736981
ACAGTAA7050.034.21306615
CAGTAAT7250.033.74316416
GGGAACC400.00298653333.5238118
GACAGGT809.1728E-833.4963761
GCTTGTA601.6408498E-533.4963761
CTGATAC400.003000262733.4924623
Produced by FastQC (version 0.11.5)