FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607892
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28050.4614306488652589No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20100.33065083929382194No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCAC19700.3240707230889697No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17380.2859060491008271No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16670.2742263428372145No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14600.24017424147710448No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT14060.23129108460055403No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13880.22833003230837054No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACCTGACCATTC13870.22816552940324925No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13220.21747284057036445No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC13210.21730833766524316No Hit
ACCCTGGACATCACCGGGCTCCAGACTGGGGACGAGGCCGATTATTACTG12890.21204424470136143No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12170.20020003553262752No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11720.1927974048021688No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11510.1893428437946214No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11370.18703980312292315No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11320.18621728859731662No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG11170.18374974502049704No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11140.18325623630513316No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10770.17716962881564488No Hit
GTCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAA10540.1733860619978549No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10390.1709185184210353No Hit
GTCTGGAGCCCGGTGATGTCCAGGGTGGCTGACGTGCCAGACTTGGAGCC10270.16894448355957967No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10190.16762846031860923No Hit
GGACATCACCGGGCTCCAGACTGGGGACGAGGCCGATTATTACTGCGACT9940.16351588769057662No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9870.1623643673547275No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9520.15660676567548182No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9500.15627775986523923No Hit
GCTCCAGACTGGGGACGAGGCCGATTATTACTGCGACTCATTGGATGACAGTTTGAATGGTCAGGTGGTATTCGG9480.1559487540549966No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9440.1552907424345114No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9240.1520006843320853No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAACAGTTCCAATATTGGGAA8950.14723010008356746No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8610.14163700130944312No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8320.1368664170609253No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8010.13176682700216485No Hit
GATTATTACTGCGACTCATTGGATGACAGTTTGAATGGTCAGGTGGTATTCGGCGGAGGGACCAAGGTGACCGTC7470.12288367012561442No Hit
GTTTGGGGGCTGTTCCTGGGAATTGCTGGTACCAGGATACATAATTATTC7440.12239016141025051No Hit
GCCCCCAAACTCCTCATTTATGACAATGATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGT7370.12123864107440137No Hit
GTGATGTCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTC7250.11926460621294573No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7170.11794858297197529No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG7160.11778408006685398No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7070.11630355392076223No Hit
CCTTGGTCCCTCCGCCGAATACCACCTGACCATTCAAACTGTCATCCAATGAGTCGCAGTAATAATCGGCCTCGT7010.11531653649003441No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6940.11416501615418527No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACCTGACCATTCA6940.11416501615418527No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGCCCGGTGATGTCCAGGGTGG6880.11317799872345745No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC6790.1116974725773657No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC6760.11120396386200179No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6740.11087495805175919No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6700.11021694643127398No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCAC6590.10840741447493962No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6440.10593987089812006No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC6330.10413033894178571No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT6320.1039658360366644No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG6290.10347232732130049No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC6250.10281431570081528No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA6240.10264981279569398No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6180.10166279536496614No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT6100.10034677212399572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4350.092.28139570
CGGGTAT453.178684E-853.33040639
TCGGGTA453.2712705E-853.13667338
TTAGAGC200.008502644549.89020512
CCTAACG200.008502644549.8902052
GTAATCA304.2794508E-449.76688462
TATGGCG605.2368705E-946.3523943
CTCTTAT3250.043.0000151
CGGATGC651.1281372E-842.4981741
TCTCGTA3350.040.78721238
CGTGAAA1400.039.2485257
TCGTATG3600.039.201740
ATGCCGT4300.038.92378244
TTTGGCG459.56967E-538.62699543
TATACAC11200.038.6055153
TTATACA11300.037.9695362
ATCTCGT3950.037.90355337
CTTATAC11350.037.8178251
ATCGCGG451.0857292E-437.80784237
CGTATGG557.3446117E-637.66882741
Produced by FastQC (version 0.11.5)