FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607892
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105421.7341896257887914No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25970.42721404460002765No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19040.31321353135096364No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18340.30169832799247237No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15910.26172412204799533No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCAC15340.252347456456081No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14590.24000973857198318No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC14350.23606166884907187No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG13630.22421745968033796No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13540.2227369335342462No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12960.21319576503721055No Hit
ACCCTGGACATCACCGGGCTCCAGACTGGGGACGAGGCCGATTATTACTG11900.1957584570943523No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG11800.19411342804313925No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11750.19329091351753272No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11750.19329091351753272No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11370.18703980312292315No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11320.18621728859731662No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC11020.1812822014436775No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10940.17996617820270705No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10140.1668059457930027No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACCTGACCATTC9970.16400939640594053No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9960.1638448935008192No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAACAGTTCCAATATTGGGAA9860.16219986444960616No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9710.1597323208727866No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9390.15446822790890485No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9320.15331670757305574No Hit
GGACATCACCGGGCTCCAGACTGGGGACGAGGCCGATTATTACTGCGACT9180.15101366690135748No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT9100.14969764366038704No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9080.14936863785014443No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8930.14690109427332487No Hit
GCTCCAGACTGGGGACGAGGCCGATTATTACTGCGACTCATTGGATGACAGTTTGAATGGTCAGGTGGTATTCGG8740.1437755390760201No Hit
GTCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAA8740.1437755390760201No Hit
GTCTGGAGCCCGGTGATGTCCAGGGTGGCTGACGTGCCAGACTTGGAGCC8550.1406499838787153No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8320.1368664170609253No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8160.13423437057898444No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8150.1340698676738631No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7840.12897027761510269No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7810.12847676889973875No Hit
GATTATTACTGCGACTCATTGGATGACAGTTTGAATGGTCAGGTGGTATTCGGCGGAGGGACCAAGGTGACCGTC7690.12650273403828313No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7240.11910010330782443No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC7060.11613905101564093No Hit
GTGATGTCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTC7040.11581004520539832No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG6990.11498753067979181No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6950.11432951905930659No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT6900.11350700453370006No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6880.11317799872345745No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6870.11301349581833615No Hit
GTTCCAATATTGGGAATAATTATGTATCCTGGTACCAGCAATTCCCAGGA6790.1116974725773657No Hit
GTTTGGGGGCTGTTCCTGGGAATTGCTGGTACCAGGATACATAATTATTC6720.11054595224151659No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC6670.10972343771591007No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6600.10857191738006093No Hit
GCCCCCAAACTCCTCATTTATGACAATGATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGT6510.1070913912339692No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCT6350.10445934475202831No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT6310.1038013331315431No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6300.1036368302264218No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG6290.10347232732130049No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACCTGACCATTCAAACTGTCATCCAATGAGTCGCAGT6230.10248530989057268No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC6120.10067577793423832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA350.003107076764.3309570
GGTATCA5350.041.6380961
AATGGTA1302.8089744E-740.41303370
GTATCAA14200.039.57021
TAGGGTC350.001442488738.88467437
TGGTATC1850.035.9293562
TAGTTCC400.002285747835.40485848
TATCAAC17300.031.8899022
TCAACGC17650.030.5118624
ATCAACG17700.030.4256693
CAACGCA18250.029.5087325
AACGCAG18600.029.1321856
TCGTATA1302.8685463E-928.56655961
CTCGTAT1302.9631337E-928.48015460
CGCAGAG19200.028.2218048
AGAGTAC19450.028.19392411
ACGCAGA19350.027.8312327
CAGAGTA19950.027.65844710
CTAGCCA2000.027.02984652
GCAGAGT20300.027.0200589
Produced by FastQC (version 0.11.5)