FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132687
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31920.2818077721382871No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24600.21718268153514608No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22340.19723012623964076No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19670.1736578595852164No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18880.1666832937960796No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18830.16624186558157725No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17710.1563538735767251No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16480.14549473949996777No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16440.14514159692836592No Hit
GTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTATATCTGCAAATGAA15010.1325167499935993No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13840.12218732977424478No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGCTTATCTTCAGCGTGAAATCTGTCTCTGCCCCACTGCCA13660.1205981882020364No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13260.11706676248601774No Hit
GTTTATTACTGCATGCAAGCTACACACTTTCCTCGCACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA12500.11035705362558235No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12490.1102687679826819No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC12420.10965076848237862No Hit
GTGTGTAGCTTGCATGCAGTAATAAACCCCGACATCCTCAGCTTCCACCCTGCTTATCTTCAGCGTGAAAT12360.10912105462497583No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12290.10850305512467256No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12170.10744362740986697No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12150.10726705612406605No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12110.10691391355246418No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12110.10691391355246418No Hit
AGCCTGGAGAGTCCCTGAGACTCACCTGTGCAGCCTCTGGATTCAACTTC11890.10497162940865394No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT11830.10444191555125115No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA11790.10408877297964926No Hit
CAGTAATGGAAACACCTTCTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGC11750.1037356304080474No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11750.1037356304080474No Hit
GTAATGGAAACACCTTCTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTAATTTATGAGATTT11660.10294105962194322No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11630.10267620269324182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)