FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908841
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATACA82580.4326185365884324No Hit
GTATTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCAGGCTCCTCATCCGTGATGCATCCAGCAGGGCCA62400.3268999356153813No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59610.3122837365710397No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCTCCCACTTTCGGCCCTGGGA57240.2998678255548786No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG51640.27053065184580594No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48330.25319028667133614No Hit
CAGTAATATAGGGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATA45320.23742155580270963No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT42910.22479609354576938No Hit
CAGTAATACACGGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCCGTCCCAGACCCACCGCCA42560.22296252018895235No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACTA41850.21924298566512349No Hit
GTATGGTAGCTCACCTCCCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT40920.21437091931700963No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37500.1964542882303974No Hit
ATATTACTGTGCGAAACCTAACTGGAACCCTCGGAGCGATTGGTTCGACCCCTGGGGCCAGGGAGCCCTGGTCAC37130.19451593925319083No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36020.18870089232157103No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG33650.17628498130540993No Hit
GCCTGGGGGGTCCCTGAGGCTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTGTGCCATGAGCTGGGTCCG33150.1736655907956713No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA33040.1730893248835288No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32880.17225111992041245No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29900.15663955248237021No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29540.15475359131535837No Hit
GTGTTAGCAGTAAGTATTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCAGGCTCCTCATCCGTGATGCAT29140.15265807890756747No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28480.14920048343471248No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28230.14789078817984316No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT28130.14736691007789543No Hit
CTATATTACTGTGCGAAACCTAACTGGAACCCTCGGAGCGATTGGTTCGA27950.14642392949438954No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC26740.14008500446082206No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT26480.13872292139575795No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGA26470.13867053358556317No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26400.13830381891419977No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26270.13762277738166773No Hit
GCCCTATATTACTGTGCGAAACCTAACTGGAACCCTCGGAGCGATTGGTT26050.13647024555738274No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCCGTGTATTACTGTCAGCAGTATGGTAGCTCA25970.13605114307582455No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25900.13568442840446115No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25700.1346366722005657No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC25500.13358891599667022No Hit
ATATAGGGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATACAGCG25460.13337936475589116No Hit
CTACCATACTGCTGACAGTAATACACGGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCCGTC25080.1313886279684898No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24980.13086474986654206No Hit
CTCCTGCGGGGCCAGTCAGAGTGTTAGCAGTAAGTATTTAGCCTGGTACC23880.12510209074511708No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23840.12489253950433796No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC23820.12478776388394842No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTGTGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG23560.12342568081888433No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC23380.12248270023537842No Hit
GTCAGAGTGTTAGCAGTAAGTATTTAGCCTGGTACCAGCAGAAACCTGGC22710.11897271695232867No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT22560.11818689979940708No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT22130.11593422396103187No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT22090.11572467272025276No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG22010.11530557023869457No Hit
GTCTGGGACGGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT21930.11488646775713639No Hit
GAGTGAAGTCCGTCCCAGACCCACCGCCACTGAATCTGTCTGGGATGCCA21690.11362916031246185No Hit
CCCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGGGGTCGAACCAATCGCTCCGAGGGTTCCAGTTAGGTTT21200.11106215761291799No Hit
TTTTAAAAGGTGTCCAGTGTGAGATACAGCTGTTGGAGTCTGGGGGAGGC21080.11043350389058074No Hit
GTGTGAGATACAGCTGTTGGAGTCTGGGGGAGGCTTGGCACAGCCTGGGGGGTCCCTGAGGCTCTCCTGTGCAGC20960.10980485016824344No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT20730.10859993053376367No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT20720.10854754272356891No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG20680.1083379914827898No Hit
CCTTTAGCAACTGTGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG20520.10749978651967346No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCTCCCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA20240.10603292783421983No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGGCATTTTAAAAGGTGTCCAGTGTGAGATACAGCTGTTGGAGT20100.105299498491493No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC20070.10514233506090868No Hit
GTAATACACGGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCCGTCCCAGACCCACCGCCA19830.10388502761623415No Hit
GTCCCAGACCCACCGCCACTGAATCTGTCTGGGATGCCAGTGGCCCTGCT19670.10304682265311778No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC19280.10100369805552165No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19190.1005322077637687No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGAG400.00706331552.28882670
GGTATCA12950.047.2493061
ATATCGT306.3855806E-445.89266637
CACGAGA4250.045.83603721
CGTATGC4200.045.26547241
TCTGGAG31800.044.50579570
ATCTCGT3800.044.38303837
TCGTATG4300.044.17064740
GTATGCC4450.043.5361642
ACGAGAC4500.043.2918622
ATGCCGT4550.041.88786744
TGCCGTC4500.040.85658645
CATCTCG4150.040.6066736
GGAGCTA4850.040.24046329
CCGTCTT4600.040.0386847
GTATCAA34650.039.757031
CGAGACG4750.039.57738523
CGAGCCC4950.039.341815
GCTACAT4900.039.20517332
TATACAC5250.039.0480423
Produced by FastQC (version 0.11.5)