FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908841
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATACA71980.3770874577819735No Hit
GTATTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCAGGCTCCTCATCCGTGATGCATCCAGCAGGGCCA61300.3211372764939563No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59020.30919285576954814No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG57510.30128229643013743No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCTCCCACTTTCGGCCCTGGGA51320.2688542419195732No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT45030.2359023093070612No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42190.22102417121174578No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACTA41390.21683314639616394No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40980.21468524617817827No Hit
CAGTAATATAGGGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATA40020.20965601639948012No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA39800.2085034845751951No Hit
CAGTAATACACGGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCCGTCCCAGACCCACCGCCA39310.20593648187565125No Hit
GTATGGTAGCTCACCTCCCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT39160.20515066472272966No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38850.2035266426066917No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36050.18885805575215536No Hit
ATATTACTGTGCGAAACCTAACTGGAACCCTCGGAGCGATTGGTTCGACCCCTGGGGCCAGGGAGCCCTGGTCAC34420.18031884269040743No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34060.1784328815233956No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG33230.17408469327722947No Hit
GCCTGGGGGGTCCCTGAGGCTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTGTGCCATGAGCTGGGTCCG32980.17277499802236018No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31200.16344996780769064No Hit
GTGTTAGCAGTAAGTATTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCAGGCTCCTCATCCGTGATGCAT30380.15915416737171928No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC29800.15611567438042245No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27880.1460572148230261No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGA26620.13945635073848475No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26430.1384609823447841No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26100.1367321846083566No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25950.135946367455435No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT25770.1350033868719291No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25690.13458428439037093No Hit
GCCCTATATTACTGTGCGAAACCTAACTGGAACCCTCGGAGCGATTGGTT25620.1342175697190075No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25380.13296026227433297No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTGTGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG25190.13196489388063226No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24940.13065519862576297No Hit
GTGTGAGATACAGCTGTTGGAGTCTGGGGGAGGCTTGGCACAGCCTGGGGGGTCCCTGAGGCTCTCCTGTGCAGC24300.1273023787732975No Hit
CTACCATACTGCTGACAGTAATACACGGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCCGTC24120.1263593981897916No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC24060.12604507132862297No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCCGTGTATTACTGTCAGCAGTATGGTAGCTCA23900.1252068663655066No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23490.12305896614752093No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23230.12169688308245684No Hit
CTATATTACTGTGCGAAACCTAACTGGAACCCTCGGAGCGATTGGTTCGA23150.12127778060089865No Hit
ATATAGGGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATACAGCG23050.12075390249895093No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC22980.12038718782758752No Hit
GTCTGGGACGGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT22930.12012524877661367No Hit
GTCAGAGTGTTAGCAGTAAGTATTTAGCCTGGTACCAGCAGAAACCTGGC22790.11939181943388685No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC22230.11645810206297959No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC22010.11530557023869457No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT21930.11488646775713639No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC21580.11305289440031935No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC21440.11231946505759255No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21430.11226707724739776No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT21350.11184797476583959No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG21190.11100976980272322No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT20970.10985723797843823No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGGCATTTTAAAAGGTGTCCAGTGTGAGATACAGCTGTTGGAGT20840.10917619644590618No Hit
CTCCTGCGGGGCCAGTCAGAGTGTTAGCAGTAAGTATTTAGCCTGGTACC20780.10886186958473754No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20680.1083379914827898No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT20100.105299498491493No Hit
TTTTAAAAGGTGTCCAGTGTGAGATACAGCTGTTGGAGTCTGGGGGAGGC20090.10524711068129824No Hit
CCCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGGGGTCGAACCAATCGCTCCGAGGGTTCCAGTTAGGTTT20010.10482800819974006No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCTCCCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA19990.1047232325793505No Hit
CCTTTAGCAACTGTGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG19940.10446129352837663No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC19850.10398980323662368No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19840.10393741542642891No Hit
GTAATACACGGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCCGTCCCAGACCCACCGCCA19810.1037802519958446No Hit
GAGTGAAGTCCGTCCCAGACCCACCGCCACTGAATCTGTCTGGGATGCCA19790.10367547637545504No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT19760.10351831294487075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)