FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336535
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCAGTTGTCAGGCTGTTCATTTGCAGAAACA80340.6011065927940533No Hit
GTATATTACTGTGCGAAGGTGTTCGTCGATAGTAGTGGCTGGGGCATTGA40690.3044439539555642No Hit
GATTATTACTGCGCCTCATATGCAGGCTTCAACAGTTATGTCTTCGGAATTGGGACCAAGGTCACCGTCTTAGGT40120.30017919470870574No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA34330.25685821920114327No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT34110.2552121717725312No Hit
GACCTAAGACGGTGACCTTGGTCCCAATTCCGAAGACATAACTGTTGAAGCCTGCATATGAGGCGCAGTAATAAT28010.20957176579737904No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC27970.2092724844467223No Hit
GTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG27850.2083746403947521No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCGTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG27140.20306239642059504No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG26990.2019400913556323No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26660.1994710202127142No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26660.1994710202127142No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC26010.19460769826454227No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25850.19341057286191532No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCGATAGTTAC24670.18458177301754164No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24040.17986809174469803No Hit
GTATATTACTGTGCGAAGGTGTTCGTCGATAGTAGTGGCTGGGGCATTGACTACTGGGGCCAGGGAACCCTGGT22200.1661011496144882No Hit
GTCTTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC21860.16355725813390595No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAACTATCGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG21210.158693936185734No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC21140.15817019382208472No Hit
GCCTTGGGCTGACCTAAGACGGTGACCTTGGTCCCAATTCCGAAGACATAACTGTTGAAGCCTGCATATGAGGCG20630.15435435660121133No Hit
GGGCTGGAGTGGGTGGCATTTATATCATATGACGGAGATATTAAATTCTA20520.15353133288690532No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20400.1526334888349351No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT19850.148518370263405No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19830.14836872958807662No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC19620.14679750249712878No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19510.14597447878282274No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCGTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC19420.14530109574384512No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19090.14283202460092703No Hit
TCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCGCCTCATAT19060.14260756358793447No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATT19040.14245792291260612No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT18830.14088669582165825No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18620.13931546873071038No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18260.13662193657479976No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18090.13534999083450863No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC18030.13490106880852354No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAAGACGGTGACCTTGGTC17910.13400322475655332No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17290.12936436382137392No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17110.12801759774341862No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16630.1244262215355378No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTACGACTCTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC16620.12435140119787359No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16100.12046074363933604No Hit
CAGTAATATACAGCCGTGTCCTCAGTTGTCAGGCTGTTCATTTGCAGAAA15790.11814131317174634No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15340.11477439797685807No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG15330.11469957763919389No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTACGACTCTGTCTCCTGGTACCAACAGCA15100.11297870987291765No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15090.11290388953525347No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14960.11193122514561908No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14670.10976143535335774No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG14530.10871395062605918No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14190.10617005914547693No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG14190.10617005914547693No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGCCCCAGCCA13940.10429955070387231No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13840.10355134732723048No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT13730.10272832361292446No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13410.10033407280767058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAACG150.002453007568.29874
TCTGGAG21350.063.57333470
GTCTAAC200.00637892553.65495364
TTAGCCC200.006541622353.31439661
GGTATCA8300.043.23011
TCGTATG2100.042.71966640
ATCTCGT1900.041.6330737
GTGGTAT1900.041.3665431
TATACAC2150.041.2968863
TAGATCT1850.040.7993134
ATGCCGT2150.040.29191644
CGTATGC2150.040.16232341
TACTAGA1900.039.64815531
ACCGTAC1900.039.58578527
GTACTAG2100.039.112730
CCGTAGT350.001412621639.054131
TCGAGCT350.001416785539.0307515
CTAGATC1950.038.67215733
GATCTCG2050.038.54608536
CGTACTA2050.038.38139729
Produced by FastQC (version 0.11.5)