FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336535
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCAGTTGTCAGGCTGTTCATTTGCAGAAACA74390.556588491883864No Hit
GTATATTACTGTGCGAAGGTGTTCGTCGATAGTAGTGGCTGGGGCATTGA38530.2882827610201005No Hit
GATTATTACTGCGCCTCATATGCAGGCTTCAACAGTTATGTCTTCGGAATTGGGACCAAGGTCACCGTCTTAGGT38400.2873100966304661No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC31000.23194304675897004No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT30950.2315689450706491No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA29880.22356316894058142No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29450.22034589442102154No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC28440.21278904031693896No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCGTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG27860.2084494607324163No Hit
GACCTAAGACGGTGACCTTGGTCCCAATTCCGAAGACATAACTGTTGAAGCCTGCATATGAGGCGCAGTAATAAT25860.1934853931995795No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG25860.1934853931995795No Hit
GTATATTACTGTGCGAAGGTGTTCGTCGATAGTAGTGGCTGGGGCATTGACTACTGGGGCCAGGGAACCCTGGT24290.18173860018630264No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22530.1685702207574063No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22400.16759755636777188No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21950.1642306411728836No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCGTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC21760.16280905475726412No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCGATAGTTAC21530.1610881869909879No Hit
GGGCTGGAGTGGGTGGCATTTATATCATATGACGGAGATATTAAATTCTA21380.15996588192602512No Hit
GTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG21130.15809537348442054No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21110.15794573280909216No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20730.1551025599778532No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAACTATCGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG20420.15278312951026346No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20170.15091262106865888No Hit
GCCTTGGGCTGACCTAAGACGGTGACCTTGGTCCCAATTCCGAAGACATAACTGTTGAAGCCTGCATATGAGGCG19870.14866801093873336No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC19770.14791980756209153No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATT19510.14597447878282274No Hit
GTCTTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC19210.14372986865289725No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC18530.13864208569173272No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAAGACGGTGACCTTGGTC18250.13654711623713559No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC17670.13220753665261292No Hit
TCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCGCCTCATAT17620.13183343496429198No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17530.13116005192531432No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17510.13101041124998597No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17210.12876580112006045No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT16880.1262967299771424No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG16560.12390247917188849No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16340.12225643174327645No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16210.12128376735364207No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16010.11978736060035838No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTACGACTCTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC16010.11978736060035838No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTACGACTCTGTCTCCTGGTACCAACAGCA15600.11671972675612685No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15370.11499885898985061No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15290.11440029628853715No Hit
CAGTAATATACAGCCGTGTCCTCAGTTGTCAGGCTGTTCATTTGCAGAAA15040.11252978784693254No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG14050.10512257441817835No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13980.10459883205452906No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13660.10220458124927517No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13650.102129760911611No Hit
GTAATATACAGCCGTGTCCTCAGTTGTCAGGCTGTTCATTTGCAGAAACAGTGTGTTCTTGGAATTGTCTCTGGA13570.10153119821029752No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT350.003447172562.6761870
TCTGGAG20450.061.14375770
CCGAAGT400.005848706254.8416670
TCGAGTC200.00708636352.24693349
GTGGTAT2500.047.730511
TGGTATC2600.045.8895652
TCGGTTT803.549287E-545.7013870
GGTATCA9100.044.5834431
GTATCAA21900.035.961341
ATTGTCT21800.035.52258764
AATTGTC22400.035.1048863
GAATTGT22600.035.01021662
GACAGGT2050.034.9247631
TGTCTCT22150.034.63702866
TTGTCTC22350.034.30495565
TTGCAGA23650.034.26957340
ATTTGCA23900.033.82438
GTGTGTT23300.033.57705351
ACAGTGT22800.033.57189648
AACAGTG23100.033.56082547
Produced by FastQC (version 0.11.5)