FastQCFastQC Report
Wed 8 May 2024
EGAF00004689629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879157
Total Bases65.1 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAATAGTTTCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGACATCA35440.40311343707665415No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32700.3719472176186961No Hit
GGATATTAGTAGGTGGATAGCCTGGTATCAGCAGAAACCAGGAAATGCGC31100.35374796538047243No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT29990.34112223414020476No Hit
AAGTAGTACTGAGTGACCACCGCCAAATCTTTCGCGCAATAATATACAGC25410.2890268746082895No Hit
GTACTACTTTGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG23450.26673279061646554No Hit
ATAATATACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATAGA22900.26047679765957615No Hit
CATCAAGATTCAGCGGCAGCGGGTCTGGGACAGATTTCAGTCTGACCATC21700.2468273584809084No Hit
CTATTAGCCTGTTGACAAAAGTAAGTAGCGAAATCTTCAGGCTGCAGGTT21000.23886518562668554No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20200.22976555950757374No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19590.22282709459175098No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19280.21930098947059515No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19040.2165711016348616No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17970.20440035170054952No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17890.2034903890886383No Hit
CATATATCCTTTGATGGAAGTCATAGAGACTATGCAGACTCCCTGAAGGG17750.20189795451779377No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC17630.20053301059992698No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17390.19780312276419343No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG16560.1883622606656149No Hit
ATCTTGATGTGACCCCGCTTTGCAAATTGGAGGCGCCAGAGATCAGCAGT16470.18733855272721484No Hit
CTCCTGTGCAGCCTCCGGATTCAGGTTCAAAAGTTATGCCATGTACTGGG16470.18733855272721484No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16000.18199252238223662No Hit
TCAGTAACCTGCAGCCTGAAGATTTCGCTACTTACTTTTGTCAACAGGCT16000.18199252238223662No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA15750.17914888922001418No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15750.17914888922001418No Hit
CTAATATCCTGACTCGCCCGACAAGAGATGTTGACCTTCTCTCCTACAGA15710.17869390791405856No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15640.1778976906286363No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT15220.1731203869161026No Hit
GCACTGAACACAGAGGGCTCACCATGGAGTTTGGGCTGACCTGGGTTTTC14920.16970802712143565No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14760.16788810189761327No Hit
GTATATTATTGCGCGAAAGATTTGGCGGTGGTCACTCAGTACTACTTTGA14740.1676606112446355No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC14710.1673193752651688No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14080.16015341969636823No Hit
GGATATATGTGCCAGCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCC13760.1565135692487235No Hit
GTACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGCTGGCACATA13750.1563998239222346No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13080.14877888704747844No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTACATGGCATAACTTT12740.1449115459468559No Hit
GATTTCAGTCTGACCATCAGTAACCTGCAGCCTGAAGATTTCGCTACTTA12680.14422907398792253No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC12680.14422907398792253No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12400.14104420484623337No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12340.1403617328873No Hit
GGGCTGGAGTGGCTGGCACATATATCCTTTGATGGAAGTCATAGAGACTA12310.1400204969078333No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT12290.1397930062548555No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12230.13911053429592213No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12220.1389967889694332No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11670.13274079601254385No Hit
GTCTCCATCTTACGTGTCTGCATCTGTAGGAGAGAAGGTCAACATCTCTT11250.1279634923000101No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC11250.1279634923000101No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC11010.1252336044642766No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11010.1252336044642766No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG10940.12443738717885429No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10810.12295869793449861No Hit
CCTTGGTCCCTTGGCCGAACGTCCGAGGGAAACTATTAGCCTGTTGACAA10650.12113877271067626No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG10570.12022881009876507No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAACTAGTGGAGTCTGGGGGAGGCG10380.118067648895476No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10290.11704394095707593No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA10270.11681645030409812No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG10250.11658895965112033No Hit
GAGTTTGGGCTGACCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCA10220.11624772367165365No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCC9940.1130628545299645No Hit
CCCTGAGACTCTCCTGTGCAGCCTCCGGATTCAGGTTCAAAAGTTATGCC9920.1128353638769867No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA9890.11249412789752002No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT9840.11192540126507552No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9800.11147041995911992No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA9680.11010547604125315No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9660.10987798538827535No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9640.10965049473529757No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9590.10908176810285307No Hit
GAACACAGAGGGCTCACCATGGAGTTTGGGCTGACCTGGGTTTTCCTCGT9570.10885427744987528No Hit
GGGTTCCCTGGCCCCAGGAGTCAAAGTAGTACTGAGTGACCACCGCCAAA9570.10885427744987528No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC9550.10862678679689748No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT9510.1081718054909419No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9470.10771682418498629No Hit
GCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA9390.10680686157307512No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG9360.10646562559360842No Hit
GCCTGGTATCAGCAGAAACCAGGAAATGCGCCTAAACTGCTGATCTCTGG9340.10623813494063063No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9290.10566940830818615No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9280.10555566298169725No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9280.10555566298169725No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9180.10441820971680826No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9070.10316701112543039No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8990.1022570485135192No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8980.1021433031870303No Hit
GATCAGCACTGAACACAGAGGGCTCACCATGGAGTTTGGGCTGACCTGGG8960.1019158125340525No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC8910.10134708590160801No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8880.10100584992214132No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA8810.10020963263671903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)