FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879157
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37390.4252937757419892No Hit
GGCTAATAGTTTCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT30620.3482881897090053No Hit
CTATTAGCCTGTTGACAAAAGTAAGTAGCGAAATCTTCAGGCTGCAGGTTACTGATGGTCAGACTGAAATCTGTC27820.31643949829211393No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27810.31632575296562504No Hit
AAGTAGTACTGAGTGACCACCGCCAAATCTTTCGCGCAATAATATACAGC20860.237272751055841No Hit
GGATATTAGTAGGTGGATAGCCTGGTATCAGCAGAAACCAGGAAATGCGCCTAAACTGCTGATCTCTGGCGCCTC20520.23340540995521847No Hit
GTACTACTTTGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTT19820.22544323710099562No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19190.21827728153219506No Hit
ATAATATACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATAGA18000.20474158768001618No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17140.194959489601971No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16070.1827887396676589No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15630.1777839453021474No Hit
CATATATCCTTTGATGGAAGTCATAGAGACTATGCAGACTCCCTGAAGGG15560.1769877280167251No Hit
CATCAAGATTCAGCGGCAGCGGGTCTGGGACAGATTTCAGTCTGACCATCAGTAACCTGCAGCCTGAAGATTTCG15550.17687398269023621No Hit
CTCCTGTGCAGCCTCCGGATTCAGGTTCAAAAGTTATGCCATGTACTGGGTCCGCCAGGCTCCAGGCAAGGGG14980.17039049908036905No Hit
ATCTTGATGTGACCCCGCTTTGCAAATTGGAGGCGCCAGAGATCAGCAGTTTAGGCGCATTTCCTGGTTTCTG14540.16538570471485753No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14270.1623145808996573No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13920.15833349447254585No Hit
CTAATATCCTGACTCGCCCGACAAGAGATGTTGACCTTCTCTCCTACAGA13800.15696855055467906No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13610.15480738935139002No Hit
TCAGTAACCTGCAGCCTGAAGATTTCGCTACTTACTTTTGTCAACAGGCT13310.1513950295567231No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12920.1469589618236561No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12870.14639023519121158No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12450.14161293147867787No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12210.13888304364294432No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12080.13740435439858864No Hit
GTACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGCTGGCACATATATCCTTTGATGGAAGTCATAGAGA11990.13638064646018855No Hit
GCACTGAACACAGAGGGCTCACCATGGAGTTTGGGCTGACCTGGGTTTTC11910.1354706838482774No Hit
GGATATATGTGCCAGCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTACATGGCATAACTTTTGAAC11620.13217206938009934No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11280.1283047282794768No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTACATGGCATAACTTT11230.12773600164703233No Hit
CCTTGGTCCCTTGGCCGAACGTCCGAGGGAAACTATTAGCCTGTTGACAAAAGTAAGTAGCGAAATCTTCAGG11160.12693978436161005No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11130.12659854838214335No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11020.12534734979076548No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10930.12432364185236539No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC10900.1239824058728987No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10880.12375491521992091No Hit
GATTTCAGTCTGACCATCAGTAACCTGCAGCCTGAAGATTTCGCTACTTACTTTTGTCAACAGGCTAATAGTTTC10770.12250371662854304No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10720.12193498999609853No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10640.12102502738418736No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10040.11420030779485348No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9990.113631581162409No Hit
GACTGAAATCTGTCCCAGACCCGCTGCCGCTGAATCTTGATGTGACCCCG9690.11021922136774205No Hit
GGTTACTGATGGTCAGACTGAAATCTGTCCCAGACCCGCTGCCGCTGAAT9580.10896802277636417No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9520.1082855508174308No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9320.10601064428765283No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG9210.10475944569627496No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9140.10396322841085266No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9100.10350824710489707No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9030.10271202981947478No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8910.10134708590160801No Hit
GTCCCAGACCCGCTGCCGCTGAATCTTGATGTGACCCCGCTTTGCAAATT8900.10123334057511911No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8870.10089210459565243No Hit
GTATATTATTGCGCGAAAGATTTGGCGGTGGTCACTCAGTACTACTTTGA8840.10055086861618574No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8830.10043712328969684No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGT200.007763391451.05350526
CGTTATT200.00782835550.946012
CGGGATC306.801495E-445.3059717
GTATCAA19550.035.9618871
GTATGGG400.002803148533.9640051
GTGGTAT2750.033.3464781
GAACGCA450.00344402232.56615466
ATCAACG21800.031.6252333
TATCAAC22200.031.3631572
CTCTAAA553.5694934E-430.8763681
AACGCAG22450.030.8626146
CAACGCA22300.030.7656025
TCAACGC22300.030.7656024
ACGCAGA23850.029.0509747
CGCAGAG24050.028.6681638
AGAGTAC24750.028.53699111
TCTCGCG4800.028.53133870
GATAATA605.943207E-428.3017273
AGGTTAC9100.027.87077146
GCAGAGT24900.027.8259329
Produced by FastQC (version 0.11.5)