FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004689631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816053
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT45890.5623409263859087No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27260.33404693077532954No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22820.2796386999373815No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCG21560.2641985263212071No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG19510.23907760892981217No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18640.22841653667102507No Hit
CAGTAATACAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATA18510.22682350288522923No Hit
GTATTACTGTGCGAGACAGTTTTACTATGATAGTAGTGGTTATTCTCCCTTTGACTACTGGGGCCAGGGAACC17190.21064808290637985No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16970.20795217957657164No Hit
GCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCACT16110.19741364837823033No Hit
GTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCA15650.19177675959772222No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15530.19030626687237226No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAAAAGCCTGGCCAGGGTCCCAGGCT15140.18552716551498494No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT14860.18209601582250173No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14490.17756199658600605No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA14130.1731505184099562No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14120.1730279773495104No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13780.1688615812943522No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13620.16690092432721895No Hit
GTCCCAGACCCACTGCCACTGAACCTGACTGGGATGCCAGTGGCCCTGTT13590.16653330114588144No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13590.16653330114588144No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13290.1628570693325066No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13200.16175419978849412No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13050.1599160838818067No Hit
GCTTATGTAGACTCTGTGAAGGGCCGATTCACCATTTCCAGAGACAACGC12490.15305378449684026No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12280.15048042222747787No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11990.14692673147454885No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC11530.14128984269404068No Hit
GCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTC11460.1404320552709199No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11450.14030951421047408No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11250.1378586930015575No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10940.13405992012773682No Hit
GTAATACAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA10920.13381483800684515No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10880.13332467376506182No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10640.13038368831436192No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10410.12756524392410787No Hit
GTTTATTACTGTCAGCAGCGTAGCACTTTCGGCCCTGGGACCAAAGTGGA10210.1251144227151913No Hit
TATGTAGACTCTGTGAAGGGCCGATTCACCATTTCCAGAGACAACGCCAA10170.12462425847340798No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10140.12425663529207048No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCAGTTTA10070.12339884786894968No Hit
GTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAAAAGCCTGGCCAGGGTCCCAGGCTCCTCATCTATGATG10020.12278614256672055No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGACTGGGATGCCA10020.12278614256672055No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9760.119600074995129No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGCTACGCTGCTGACAGTAATAAACTG9700.11886482863245403No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9500.11641400742353746No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9490.11629146636309162No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9490.11629146636309162No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9450.1158013021213083No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC9290.11384064515417504No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT9260.11347302197283755No Hit
GTCTGAGAGCCGAGGACACGGCCTTGTATTACTGTGCGAGACAGTTTTAC9230.11310539879150006No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGTCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT9220.11298285773105422No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8960.10979679015946268No Hit
GTATTAATCGGAATGGTGGTAGCACAGCTTATGTAGACTCTGTGAAGGGCCGATTCACCATTTCCAGAGACAACG8960.10979679015946268No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT8950.10967424909901687No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8950.10967424909901687No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8940.10955170803857103No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC8880.10881646167589605No Hit
GCCTGGGACCCTGGCCAGGCTTTTGTTGGTACCAGGCTAAGTAGCTGCTA8880.10881646167589605No Hit
CTGCTACTCTGGCTCCCAGATACCGCCGGAGAAATTGTGTTGACACAGTC8840.10832629743411273No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8740.10710088682965445No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8670.10624309940653363No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG8570.10501768880207536No Hit
GTAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA8570.10501768880207536No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8530.10452752456029205No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8440.10342465501627957No Hit
GTGCTACGCTGCTGACAGTAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC8400.10293449077449628No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8380.10268940865360461No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8360.10244432653271295No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8340.1021992444118213No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGTCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA8320.10195416229092963No Hit
GTGTTAGCAGCTACTTAGCCTGGTACCAACAAAAGCCTGGCCAGGGTCCC8250.10109637486780884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)