FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816053
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95561.171002373620341No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29310.3591678481667245No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCG20080.24606244937522442No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19870.243489087105862No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG18260.22375997637408357No Hit
CAGTAATACAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATA17520.21469193790109223No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17160.2102804597250424No Hit
GTATTACTGTGCGAGACAGTTTTACTATGATAGTAGTGGTTATTCTCCCTTTGACTACTGGGGCCAGGGAACC16260.19925176428491775No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAAAAGCCTGGCCAGGGTCCCAGGCT15740.19287962914173465No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT15730.19275708808128883No Hit
GCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCACT15600.19116405429549307No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15480.1896935615701431No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15390.18859069202613066No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15330.18785544566345566No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA14570.17854232506957268No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13500.16543043160186902No Hit
GTCCCAGACCCACTGCCACTGAACCTGACTGGGATGCCAGTGGCCCTGTT13090.16040624812359003No Hit
GTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCA13060.16003862494225252No Hit
GTGCTACGCTGCTGACAGTAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC12820.15709763949155262No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12710.15574968782664853No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12620.15464681828263604No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11950.14643656723276552No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11600.1421476301171615No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11390.13957426784779908No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11360.1392066446664616No Hit
GCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTC11230.13761361088066584No Hit
GCTTATGTAGACTCTGTGAAGGGCCGATTCACCATTTCCAGAGACAACGC11210.13736852875977418No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11190.1371234466388825No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11090.13589803603442424No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11030.13516278967174924No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10960.13430500224862846No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10940.13405992012773682No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGCTACGCTGCTGACAGTAATAAACTG10780.13209926316060355No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10540.12915827770990365No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGACTGGGATGCCA10450.12805540816589117No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10420.12768778498455371No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10410.12756524392410787No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10260.12572712801742042No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10070.12339884786894968No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCAGTTTA10060.12327630680850386No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10010.12266360150627471No Hit
GTTTATTACTGTCAGCAGCGTAGCACTTTCGGCCCTGGGACCAAAGTGGA9820.12033532135780398No Hit
GTCTGAGAGCCGAGGACACGGCCTTGTATTACTGTGCGAGACAGTTTTAC9670.11849720545111654No Hit
GTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAAAAGCCTGGCCAGGGTCCCAGGCTCCTCATCTATGATG9640.11812958226977904No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT9620.11788450014888739No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC9500.11641400742353746No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9470.11604638424219996No Hit
TATGTAGACTCTGTGAAGGGCCGATTCACCATTTCCAGAGACAACGCCAA9440.11567876106086247No Hit
GCCTGGGACCCTGGCCAGGCTTTTGTTGGTACCAGGCTAAGTAGCTGCTA9420.11543367893997081No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9380.11494351469818749No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT9170.11237015242882507No Hit
GTATTAATCGGAATGGTGGTAGCACAGCTTATGTAGACTCTGTGAAGGGCCGATTCACCATTTCCAGAGACAACG9060.11102220076392097No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9040.1107771186430293No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9040.1107771186430293No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8720.1068558047087628No Hit
GTAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA8710.10673326364831696No Hit
GTAATACAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA8660.10612055834608781No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGTCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT8500.10415990137895455No Hit
CTGCTACTCTGGCTCCCAGATACCGCCGGAGAAATTGTGTTGACACAGTC8420.10317957289538793No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC8390.10281194971405044No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8380.10268940865360461No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8270.10134145698870048No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8220.10072875168647136No Hit
GTGTTAGCAGCTACTTAGCCTGGTACCAACAAAAGCCTGGCCAGGGTCCC8210.10060621062602552No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGTCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA8180.10023858744468803No Hit
GCCTTGTATTACTGTGCGAGACAGTTTTACTATGATAGTAGTGGTTATTC8170.10011604638424221No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8170.10011604638424221No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)