FastQCFastQC Report
Wed 8 May 2024
EGAF00004689633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538904
Total Bases113.5 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTG120680.7841944656716728No Hit
GTTTTATACAGCTCCAACAACAAGAACTACTTAGCTTGGTACCAGCAGAA116770.758786772924107No Hit
ATATTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA83120.5401246601477415No Hit
GGGTAATATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGC75100.4880096484251129No Hit
CTCCTAAGGTGCTCATTTACTGGGCATCTGCCCGGGAATCCGGGGTCCCT74300.48281114351512505No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA69430.4511652448755738No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC68150.4428476370195932No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT67650.4395985714508508No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG56900.3697436617228885No Hit
ATATTATATTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA56130.3647401007470252No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46180.3000836959290508No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC44260.28760728414507986No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44130.28676252709720684No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG41820.27175184416961684No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA41000.26642337663687926No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG40100.26057505861314284No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA39400.25602636681690344No Hit
ATATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT38270.24868347863154558No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT37670.24478459994905466No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT37210.24179545962581162No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC37140.2413405904461877No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA37040.24069077733243918No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC36530.23737673045232194No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA36250.23555725373382613No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC36040.2341926461949543No Hit
GTTTATTACTGTCAACAATATTATATTACCCCGCTCACTTTCGGCGGAGG34800.22613496358447313No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT34330.22308084194985522No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC33750.219311925890114No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG32950.21411342098012612No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32630.21203401901613095No Hit
AACCAGGACAGCCTCCTAAGGTGCTCATTTACTGGGCATCTGCCCGGGAA32210.2093048039383873No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC30870.2005973082141576No Hit
GTCAGGGACCCCGGATTCCCGGGCAGATGCCCAGTAAATGAGCACCTTAG29750.19331940134017456No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC29090.18903063478943455No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA29010.18851078429843576No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG28700.18649636364581548No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTA27180.17661920431683847No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT27120.17622931644858938No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC27120.17622931644858938No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG27030.17564448464621576No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTT26860.17453980235284333No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA26310.17096583022722664No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26050.1692763161314806No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCC25740.16726189547886028No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC25310.1644676990897418No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTGTTGTTGGAGCTG25160.16349297941911908No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24690.16043885778450118No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT24680.16037387647312634No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGGTGCTCA24280.1577746240181324No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23210.1508216237010236No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA23130.1503017732100248No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA23030.1496519600962763No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG22620.1469877263299075No Hit
CATTTACTGGGCATCTGCCCGGGAATCCGGGGTCCCTGACCGATTCAGTG22250.14458341780903813No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC22200.14425851125216388No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC22180.14412854862941418No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21930.14250401584504296No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG21520.13983978207867417No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA21480.13957985683317478No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21450.13938491289905022No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21410.13912498765355086No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTC21280.13828023060567782No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC20420.13269183782744082No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20370.1323669312705666No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC20350.1322369686478169No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG20210.13132723028856902No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT19920.1294427722586984No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCC19910.12937779094732355No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC19690.12794820209707686No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC19670.1278182394743272No Hit
GAATCGGTCAGGGACCCCGGATTCCCGGGCAGATGCCCAGTAAATGAGCA19540.12697348242645415No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19180.1246341552169596No Hit
CTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA19070.12391936079183627No Hit
GCCTCCTAAGGTGCTCATTTACTGGGCATCTGCCCGGGAATCCGGGGTCC18980.12333452898946264No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA18700.12151505227096686No Hit
GTAAATGAGCACCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA18690.12145007095959202No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18530.12041036997759444No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT18460.11995550079797049No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT18390.11950063161834656No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAACAA18190.1182010053908496No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC18010.11703134178610232No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGGTGCTCATT17870.11612160342685444No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17850.11599164080410473No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT17750.11534182769035627No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17750.11534182769035627No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17650.11469201457660777No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAAC17610.11443208933110836No Hit
GCTAAGTAGTTCTTGTTGTTGGAGCTGTATAAAACACTCTGGCTGGACTT17390.1130025004808617No Hit
CTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAACAAGAACTACT17310.11248264998986292No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCT17130.11131298638511564No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT17110.11118302376236595No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATATTACCCCGC17050.11079313589411686No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17020.11059819195999232No Hit
GCAGAAACCAGGACAGCCTCCTAAGGTGCTCATTTACTGGGCATCTGCCC17000.1104682293372426No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG16860.10955849097799472No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGGTGC16710.108583771307372No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16710.108583771307372No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC16610.10793395819362352No Hit
GTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG16580.10773901425949897No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC16520.10734912639124988No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16480.10708920114575048No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA16410.10663433196612654No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGCAGATGCCCAGTAAAT16260.10565961229550382No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA16250.10559463098412897No Hit
GCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGG16060.10435998606800684No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGT15940.10358021033150867No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGCGGGGTAATATAATATTGTTGACAG15880.10319032246325957No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15860.10306035984050986No Hit
GTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGCTCATTTGCAGATACA15820.10280043459501048No Hit
GCCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGAC15820.10280043459501048No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15770.10247552803813623No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG15720.10215062148126199No Hit
CAGTAAATGAGCACCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGC15650.10169575230163803No Hit
GCTCCAACAACAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAG15620.10150080836751349No Hit
GCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGGTGCTCATTTACTG15580.10124088312201411No Hit
AGTCCAGCCAGAGTGTTTTATACAGCTCCAACAACAAGAACTACTTAGCT15490.10065605131964048No Hit
GCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAG15480.10059107000826562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)