FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867988
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29060.3347972552615935No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT28600.3294976428245552No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27420.31590298483389173No Hit
CAATATAACAGTCACTCTACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT23330.26878251773065986No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGCCTCCTCACCTCCATGTAGGCTGTGCTGATGGACGTGTCCCGGGT22800.26267644253146355No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20740.23894339553081378No Hit
CTCTACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTG20650.23790651483661063No Hit
GTTATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC18890.217629736816638No Hit
AACGTAGAGTGACTGTTATATTGTTGGCAGTAATAAGTTGCAAAATCATC18810.21670806508845747No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18510.21325179610778028No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGACTTGAGTGGATGGGATGGATCAACCCTAACAGTGGTGA17780.20484154158813256No Hit
GAATTCACCCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAATATAACAGTCA17400.20046360087927484No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16890.19458794361212367No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16390.18882749531099507No Hit
GTGTATTACTGTGCGAGAGCCGGACTCATCTTCGACTACTTCCTTGACTA16360.18848186841292738No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15860.1827214201117988No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15510.17868910630100876No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15340.17673055387862505No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG15260.1758088821504445No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15190.17500241938828648No Hit
ATATAACAGTCACTCTACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT14720.1695875979852256No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA14540.16751383659681932No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14500.16705300073272902No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14340.16520965727636788No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAAAAA14190.16348152278602932No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14150.16302068692193902No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13600.15668419379069756No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13450.154956059300359No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12960.14931081996525297No Hit
CTGTTATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGGGTGAATTCTGTC12880.14838914823707242No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG12800.14746747650889183No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCT12550.14458725235832753No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12390.14274390890196637No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12150.13997889371742467No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA11780.13571616197458952No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT11550.13306635575607037No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11430.1316838481637995No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11330.1305317585035738No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10820.12465610123642262No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10560.12166066811983575No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10360.11935648879938432No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10310.11878044396927147No Hit
GGGTGCGACAGGCCCCTGGACAAGGACTTGAGTGGATGGGATGGATCAACCCTAACAGTGGTGACACAATGTATG10290.11855002603722632No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10280.11843481707120374No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10230.1178587722410909No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10130.11670668258086517No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT10130.11670668258086517No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10110.11647626464882004No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC10110.11647626464882004No Hit
CCCTAACAGTGGTGACACAATGTATGCACAGAAGTTTCAGGACTGGGTCACCATGACCCGGGACACGTCCATCAG10020.11543938395461688No Hit
GCCTACATGGAGGTGAGGAGGCTGAGATCTGACGACACGGCCGTGTATTA9990.11509375705654916No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9730.1120983239399623No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9600.11060060738166888No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9590.1104853984156463No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9520.1096789356534883No Hit
GTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACCAGCTACTA9450.10887247289133029No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9380.10806601012917229No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9340.10760517426508201No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACC9340.10760517426508201No Hit
CAGTAATACACGGCCGTGTCGTCAGATCTCAGCCTCCTCACCTCCATGTA9300.10714433840099172No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9220.10622266667281113No Hit
GTATTACTGTGCGAGAGCCGGACTCATCTTCGACTACTTCCTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT9080.10460974114849514No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8960.10322723355622428No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8680.10000138250759226No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGGT150.002377040568.8406338
TCGTATG2250.056.74411440
ATCTCGT2100.052.3921437
CATCTCG2100.052.3495936
CGTATGC2500.051.12928841
TCTCCGA2700.050.54649711
CGAGACG2800.047.5501323
GGAGCTA2600.047.3451929
CACGAGA2900.047.0713821
ATGCCGT2750.046.6198744
CCGTCTT2800.045.97297747
AGCTACA2750.044.81447631
ACGAGAC3050.044.75897622
GTGGTAT2300.044.5182841
GCCGTCT2950.043.5867146
ATCTCCG3150.043.3255710
TACATCT2650.042.72397234
CTTGAAA2900.042.53814757
CTACATC2750.042.38603633
TACACAT3250.041.9876375
Produced by FastQC (version 0.11.5)