FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699701
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCGGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT63190.3717712703587278No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58620.3448841884543223No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54470.3204681293945229No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAATTCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT54170.31870311307694704No Hit
CAGTAATACAAGGCGGTGTCCTCAGTTCTCAGACTGATCATTTGCAGATA50170.29516956217593565No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCG43460.255692030539489No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG40930.24080705959459928No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36160.21274330014514317No Hit
GTATTACTGTGCCCGATCTGGGTATGATCGTAGTGCTTTTTTTCACTACTGGGGCCGGGGAACCCTGGTCACCGT34710.20421238794352653No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33340.19615214675993012No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32390.19056292842093991No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGAATTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG32140.18909208148962672No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31700.18650339089051546No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA27320.16073415265390795No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27060.1592044718453422No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT26920.15838079756380682No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGAAT26730.15726295389600875No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT26460.1556744392101905No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT26270.15455659554239246No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTA25450.14973221760768513No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25300.1488497094488972No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25040.14732002864033145No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC24900.14649635435879604No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24450.14384882988243225No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23900.1406129666335432No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23500.13825961154344205No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23060.1356709209443308No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22950.135023748294553No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTCGGCTCACTTTCGGCGG22860.13449424339928023No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG22210.13067004137786586No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA22000.1294345299555628No Hit
GTAATAAACTGCAAATTCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA21800.1282578524105122No Hit
TGATTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGTCTGGAGT21430.12608099895216865No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC21250.12502198916162313No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21240.12496315528437062No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC21180.12461015202085543No Hit
GTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG20700.12178612591273406No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGATGATTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGT20690.12172729203548154No Hit
GTATGTACTACCACCATCCCAACTAATAAGAGAGACCCACTCCAGACCCTTCCCCGGAGCTTGACGGACCCAGTG20480.12049178061317845No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20040.11790309001406718No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG19110.11243153942958202No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT18750.110313519848491No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18540.1090780084261879No Hit
GGATACCAGTGGCCCTGGTGGATGCATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG18340.10790133088113733No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18030.10607748068630894No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17740.10437129824598561No Hit
GCACTGAACACAGAGAACGCACCATGGAGTCTGGACTGACCTGGGTTTTC17550.10325345457818758No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17160.10095893336533895No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC17040.10025292683830862No Hit
CCCAGATACCGCTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCAC17030.10019409296105608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGA300.001828436373.5482670
GTGGTAT3300.045.410321
GGTATCA13500.044.1489221
CCGCACA350.00143459138.9322974
GTATCAA34750.038.1250651
TGTAACC400.002765854834.05874312
TATCAAC39750.032.902032
ATCAACG40550.032.0887073
ACGGGAT851.3452518E-732.07794616
TCAACGC40750.031.850444
TAATGTC450.003983622431.6142262
CAACGCA41250.031.46535
AACGCAG41650.031.1631136
CGCGTCA450.00468060630.58952338
GACAGGA2800.030.4086951
GACAGGT2850.029.8752121
ACGCAGA44050.029.4652357
CGCAGAG44700.028.8843468
AGAGTAC45000.028.53449611
AGTCTAT605.8434554E-428.3856263
Produced by FastQC (version 0.11.5)