FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046450
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGGAGGGCCACCGGGAACGTTCGGCCAAGGGA53330.5096277891920302No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30330.2898370681829041No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26650.25467055282144396No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG26110.24951024893688184No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA25300.2417697931100387No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20890.19962731138611495No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19960.19074012136270246No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19820.18940226480003822No Hit
GTATGGAGGGCCACCGGGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT19310.18452864446461847No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT18490.17669262745472789No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18250.17439915906158918No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17910.171150078837976No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17470.1669453867838884No Hit
GCCTAAGAGGGGAGGACACGGCTGTGTTTTACTGTGCGAAGGATCATATTTCGGTGGACCTAAGTACTGGCTGGA16580.15844044149266567No Hit
GTCTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCACCTGCAGGGCCAGTCAATTTGTTGGGAG16220.1550002389029576No Hit
GTCCCAGACCCACCGCCACTGAATCTGTCTGGTATGCCAGCGGCCCTGCT16170.1545224329877204No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15830.15127335276410722No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14960.14295952983897942No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14910.1424817239237422No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14730.14076162262888814No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14700.14047493907974581No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14480.138372593052702No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14360.13722585885613264No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13990.13369009508337715No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13940.1332122891681399No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13580.12977208657843184No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13360.12766974055138802No Hit
CTCCCAGACTCCTCATCTTTGATGCGTCCAGCAGGGCCGCTGGCATACCA12890.12317836494815806No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAATGCATGGCATAACTAC12810.1224138754837785No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12520.11964260117540255No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12230.11687132686702661No Hit
CCTCCATACTGCTGACAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC11930.11400449137560321No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11800.11276219599598643No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATGCCATGCATTGGGTCCGCCAGGCTCCAGGCAAGGGG11620.1110420947011324No Hit
GTCTGGGAGCCTGGCCAGATTTCTGTTGGTACCAGGCTAAGAAGTTGCTC11200.10702852501313966No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCACCTGCAGGGCCAGTCAAT11170.10674184146399733No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG11040.10549954608438052No Hit
TCTTTGATGCGTCCAGCAGGGCCGCTGGCATACCAGACAGATTCAGTGGCGGTGGGTCTGGGACAGACTTCACT10900.10416168952171627No Hit
GTGTATTATTGTCAGCAGTATGGAGGGCCACCGGGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA10850.10368388360647905No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC10770.10291939414209948No Hit
GAGTGAAGTCTGTCCCAGACCCACCGCCACTGAATCTGTCTGGTATGCCA10770.10291939414209948No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC10760.10282383295905204No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10680.10205934349467247No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTATTGTCAGCAGTATGGAGGGCCA10660.10186822112857757No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT200.006119915354.2192565
CGACCGA200.00719902352.03813645
GTCGTGA306.596808E-445.5886331
GGTATCA7250.043.2674941
AACTGTG19400.041.34234670
GTATCAA20200.040.8484841
TATCAAC22950.035.9348872
ATCAACG23150.035.6244353
TCAACGC23450.035.1686864
CAACGCA24150.034.2904175
CGTACTA1400.034.14237229
AACGCAG24500.033.939656
GACCGTA1450.032.9287926
TCGTATG1600.032.30540540
ACGCAGA26050.031.9186827
CGTATGC1650.031.37648241
ACGAGAC1750.031.16664722
CGCAGAG26650.031.0721938
ATCTCGT1550.031.01346437
TGTACGA450.0045881630.71341542
Produced by FastQC (version 0.11.5)