FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046450
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGGAGGGCCACCGGGAACGTTCGGCCAAGGGA51510.492235653877395No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28950.2766496249223565No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27840.26604233360409No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA25740.24597448516412634No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22030.21052128625352381No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20540.19628266997945434No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA20040.19150461082708203No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19570.18701323522385208No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA19350.18491088919680826No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT18750.1791772182139615No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18240.17430359787854172No Hit
GTATGGAGGGCCACCGGGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT17950.1715323235701658No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17850.17057671173969133No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16710.15968273687228246No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15690.14993549620144297No Hit
GTCCCAGACCCACCGCCACTGAATCTGTCTGGTATGCCAGCGGCCCTGCT15090.1442018252185962No Hit
GCCTAAGAGGGGAGGACACGGCTGTGTTTTACTGTGCGAAGGATCATATTTCGGTGGACCTAAGTACTGGCTGGA15010.14343733575421663No Hit
GTCTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCACCTGCAGGGCCAGTCAATTTGTTGGGAG14680.1402838167136509No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14680.1402838167136509No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13490.1289120359310048No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13120.1253762721582493No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13010.1243250991447274No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13000.12422953796167996No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12960.12384729322949019No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12770.12203163075158871No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC12120.1158201538535047No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAATGCATGGCATAACTAC12110.11572459267045726No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12010.11476898083998278No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC11920.1139089301925558No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11830.11304887954512878No Hit
CTCCCAGACTCCTCATCTTTGATGCGTCCAGCAGGGCCGCTGGCATACCA11810.11285775717903387No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATGCCATGCATTGGGTCCGCCAGGCTCCAGGCAAGGGG11620.1110420947011324No Hit
CCTCCATACTGCTGACAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC11600.11085097233503752No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11520.11008648287065793No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11430.10922643222323093No Hit
GTACTTAGGTCCACCGAAATATGATCCTTCGCACAGTAAAACACAGCCGTGTCCTCCCCTCTTAGGCTGTTCAT11370.10865306512494624No Hit
GTCTGGGAGCCTGGCCAGATTTCTGTTGGTACCAGGCTAAGAAGTTGCTC11280.10779301447751923No Hit
TCTTTGATGCGTCCAGCAGGGCCGCTGGCATACCAGACAGATTCAGTGGCGGTGGGTCTGGGACAGACTTCACT11280.10779301447751923No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11140.10645515791485498No Hit
GTGTATTATTGTCAGCAGTATGGAGGGCCACCGGGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA11120.10626403554876009No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCACCTGCAGGGCCAGTCAAT11070.10578622963352285No Hit
GTGTTTTACTGTGCGAAGGATCATATTTCGGTGGACCTAAGTACTGGCTGGACCAACTTGTTTGAGTCCTGGGGC10780.10301495532514693No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10780.10301495532514693No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTATTGTCAGCAGTATGGAGGGCCA10700.10225046586076736No Hit
GAGTGAAGTCTGTCCCAGACCCACCGCCACTGAATCTGTCTGGTATGCCA10620.10148597639638779No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC10500.10033924219981843No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC10490.10024368101677099No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGCC150.002421771268.5187335
TACGGAA306.763619E-445.358162
GGTATCA8450.042.6762851
AACTGTG19500.042.06603270
GTATCAA20250.036.960271
CGGCAAA1051.9363312E-434.26431370
CGGGATT400.002782049134.0169944
GATGGAG8750.031.24905270
ATCAACG23850.030.9489943
CGCACTT450.004620154430.66981341
AACGCAG24500.030.5458726
TATCAAC24200.030.50432
TCAACGC24300.030.3773164
CAGGTAT1251.4733814E-1029.9378191
CAACGCA25000.029.7988855
GACAGGT1600.029.7677151
GAACTGT17250.029.20744169
ACGCAGA26200.028.5638877
CGCAGAG26100.028.5429948
AGAGTAC26800.027.91772511
Produced by FastQC (version 0.11.5)