FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054475
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGG59070.5601839778088622No Hit
CTATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGACGGTGAGAGTGAATTCTGTC56100.5320183029469641No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47020.445909101685673No Hit
GAGTATCAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGTGT43060.40835486853647546No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42270.4008629886910548No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTGATACTCTGA33520.3178833068588634No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31240.2962611726214467No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC29460.2793807344887266No Hit
TCTATAAGGTGTCTAATTTAGAAAGTGGAGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT29290.27776855781312976No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29030.27530287583868746No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27090.2569050949524645No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26830.25443941297802225No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA26560.25187889708148603No Hit
GAATTCACTCTCACCGTCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA26500.2513098935489224No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26260.24903387941866809No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATCAGTAGCTGGTTGGCCTGGTATCAGCAGAAA26220.24865454373029233No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25400.24087816211858984No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA24960.23670546954645677No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24260.23006709499988148No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23870.22636857203821806No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23800.22570473458356052No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23630.22409255790796367No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22730.2155575049195097No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22120.20977263567177978No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21640.20522060741127104No Hit
GGCCAAAAGAGTACCCCGGAGAATAACTATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGG21470.20360843073567417No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT21260.20161691837170154No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT19970.1893833424215842No Hit
ACTTATTACTGCCTACACTATAATAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAA19750.18729699613551767No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTGCCACC19290.18293463571919674No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19010.18027928590056663No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18200.17259773821095806No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC17720.16804570995044926No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17460.16558002797600702No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16780.15913132127361956No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGACGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG16730.1586571516631499No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16610.15751914459802271No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTG16150.15315678418170178No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16120.15287228241542No Hit
CTGATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA15430.1463287417909386No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14660.13902652978970578No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14580.1382678584129543No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14570.13817302449086039No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14460.13712985134782713No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14330.13589701036060597No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14210.1347590032954788No Hit
ACCCAGTCTCCTGCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT14080.13352616230825765No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13930.13210365347684866No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13670.1296379715024064No Hit
CCCCTAAACTCCTGATCTATAAGGTGTCTAATTTAGAAAGTGGAGTCCCA13630.1292586358140307No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13420.12726712345005808No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13340.1265084520733066No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13060.1238531022546765No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12980.12309443087792503No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACTCCA12930.12262026126745537No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12690.12034424713720096No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12670.12015457929301311No Hit
CTCCTGATCTATAAGGTGTCTAATTTAGAAAGTGGAGTCCCATCAAGGTT12640.1198700775267313No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGAG12550.1190165722278859No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12490.11844756869532232No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGTGTCTAATTTAGAAAGTGGAGTCCCAT12320.11683539201972545No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12110.11484387965575286No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12080.11455937788947106No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11990.11370587259062566No Hit
TAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT11850.1123781976813106No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11810.11199886199293486No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11580.10981768178477441No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11510.10915384433011688No Hit
ATAATAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT11410.10820550510917755No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11340.10754166765452002No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11280.10697266412195643No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11200.10621399274520496No Hit
GTGGAGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT11140.10564498921264137No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC11080.10507598568007775No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11010.10441214822542023No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10980.10412764645913843No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10900.10336897508238696No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10900.10336897508238696No Hit
ACCTTGATGGGACTCCACTTTCTAAATTAGACACCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG10780.10223096801725978No Hit
GTCTCCTGCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATCAGTAG10660.10109296095213258No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10660.10109296095213258No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10570.10023945565328718No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGAAT350.00466518658.06476670
ATGCCGT5300.047.7780244
CGTATGC5500.047.76450341
TCGTATG5400.046.6748240
GACCGGA6000.046.0929626
ATCTCGT4800.045.87463437
ACGAGAC5950.045.8650822
TATCGCG306.4253475E-445.8329636
GCCTATC5200.045.51251633
AGACCGG6100.045.32872825
CACGAGA5950.045.2874721
GAGCCTA6000.045.0811631
GCCGTCT5200.044.7807646
ATCTCCG6200.044.54474610
ACCGGAG6300.044.45478427
CCCACGA6150.043.80847519
TATACAC6150.043.796013
AGCCTAT5750.043.49538832
GAGACCG6400.043.19368724
TCTCCGA6350.042.95556311
Produced by FastQC (version 0.11.5)