FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054475
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGACGGTGAGAGTGAATTCTGTC57810.5482349036250267No Hit
CTATAATAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGG56030.5313544654923066No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48680.46165153275326587No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47590.45131463524502713No Hit
GAGTATCAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGTGT39300.37269731382915666No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34410.32632352592522346No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTGATACTCTGA32250.3058393987529339No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31790.301477038336613No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30680.2909504729841864No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27410.25993978045947036No Hit
TCTATAAGGTGTCTAATTTAGAAAGTGGAGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT26210.2485597098081984No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26130.24780103843144693No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA26110.24761137058725907No Hit
GAATTCACTCTCACCGTCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA25620.24296450840465633No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25080.23784347661158395No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25020.23727447307902036No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23850.2261789041940302No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATCAGTAGCTGGTTGGCCTGGTATCAGCAGAAA23540.22323905260911828No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA22650.21479883354275825No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22230.21081580881481304No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21730.20607411271011644No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21510.2039877664240499No Hit
GGCCAAAAGAGTACCCCGGAGAATAACTATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGG20590.19526304559140806No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACTCCA20260.19213352616230825No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20120.19080585125299318No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19950.18919367457739633No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19360.1835984731738543No Hit
ACTTATTACTGCCTACACTATAATAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAA19310.18312430356338463No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGACGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG19060.1807534555110363No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19010.18027928590056663No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18820.1784774413807819No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18170.17231323644467625No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17970.17041655800279762No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17130.1624505085469072No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT17100.16216600678062543No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTGCCACC16770.15903648735152565No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC16520.15666563929917732No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16290.15448445909101685No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15540.1473719149339719No Hit
CTGATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA15460.1466132435572204No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15450.1465184096351265No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTG15380.14585457218046896No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14810.14044903862111477No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14810.14044903862111477No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14460.13712985134782713No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14290.13551767467223025No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13850.1313449821000972No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13780.13068114464543967No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13750.13039664287915786No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13360.1266981199174945No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13160.12480144147561582No Hit
ACCCAGTCTCCTGCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT12880.1221460916569857No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGAG12820.1215770881244221No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12750.12091325066976458No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12530.11882690438369806No Hit
CTCCTGATCTATAAGGTGTCTAATTTAGAAAGTGGAGTCCCATCAAGGTT12510.11863723653951018No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12420.11778373124066478No Hit
CCCCTAAACTCCTGATCTATAAGGTGTCTAATTTAGAAAGTGGAGTCCCA12040.11418004220109534No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11880.11266269944759241No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11620.11019701747315015No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG11560.10962801394058656No Hit
ATAATAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT11460.1086796747196472No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11370.10782616942080182No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11310.10725716588823822No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11130.10555015529054744No Hit
TAGTTATTCTCCGGGGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT11080.10507598568007775No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11040.10469664999170204No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11030.1046018160696081No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10910.1034638090044809No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10910.1034638090044809No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGTGTCTAATTTAGAAAGTGGAGTCCCAT10850.1028948054719173No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10820.10261030370563552No Hit
ATTATAGTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC10790.10232580193935371No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10630.10080845918585077No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10600.10052395741956897No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)