FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865835
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64740.34697601877979567No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60080.3220006056269713No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49330.2643856503924516No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43870.23512261266403514No Hit
GTTTAGGGGCATTCCCTGGCTTTTGTTGATACCAATTTAAATAAGTCTCG43410.23265722853306964No Hit
CCCCTAAACTCCTCATCTATGGTGCATCTAATTTACTAAGTGGGGTCCCT41430.22204535770847905No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40760.2184544721264206No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG38250.2050020500205002No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38220.20484126409891548No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34650.1857077394303355No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34060.18254561630583627No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33780.181044947704379No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33240.1781508011158543No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33180.17782922927268488No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33030.17702529966476135No Hit
AGTCAAGACATCGAGACTTATTTAAATTGGTATCAACAAAAGCCAGGGAA32670.1750958686057449No Hit
GTTCAGTGCCACTGGATCTGGGACAGATTTCACTCTCACCATCATCAGTCTGCAACCTGAAGATTTTGCAACTTA32550.17445272491940605No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31220.1673245490624841No Hit
GTGCAGCCACAGTTCGTTTAATCTCCACTTTGGTCCCTCCGCCGAAAGCG30450.16319771040847664No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAAGACATCGAGACTTATTTAAATTGGTATCAACAAAAG30110.16137546996384997No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTTTGG29970.16062513566312134No Hit
ACTTACTACTGTCAACAGAGTCGCAGTCTCTTTTCGCTCGCTTTCGGCGG28870.1547296518716821No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28700.1538185316493688No Hit
GATTTCACTCTCACCATCATCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTCGCAGTCT27730.1486197868514633No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26860.1439569951255068No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26830.1437962092039221No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26390.1414380156873464No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT26100.13988375177869428No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25950.13907982217077072No Hit
GGGTACGCCAGTCCCCAGGGAAGAGACTGGAGTGGATTGCGAGCATCTATAATAGTGGCAGCATCTATAACAACC25860.1385974644060166No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT25580.13709679580455936No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTTTGGCTCCGAGGTGCCAGATGTGACAT25300.1355961272031021No Hit
GAAGTATGTAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCCGCAGGGAGAACTGGTTCTTGTCCGTGTCCAAGGA25280.13548893658871228No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT25060.13430983983042444No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGATGATGGTGAGAGTGAAATCTGTCCCAGATCCAGTGGCA24920.1335595055296958No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24810.1329699571505519No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCACTTTGGTCC24440.13098693078434054No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24260.1300222152548323No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23550.12621694844399425No Hit
CCATAGATGAGGAGTTTAGGGGCATTCCCTGGCTTTTGTTGATACCAATT23430.12557380475765542No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG23360.1251986376072911No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG23270.12471627984253698No Hit
CATCTATGGTGCATCTAATTTACTAAGTGGGGTCCCTTCGCGGTTCAGTGCCACTGGATCTGGGACAGATTTCA23170.12418032677058796No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC23100.12380515962022365No Hit
CACCATCATCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTCGCAGTCTCTTTTCGCTCG22870.12257246755474091No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22480.12048225057413972No Hit
GTCTTGACTTGCCCGGCAAGTGATGGTGACT21970.11774888990719971No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21960.11769529460000483No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT21870.11721293683525069No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21870.11721293683525069No Hit
GAGTGAAATCTGTCCCAGATCCAGTGGCACTGAACCGCGAAGGGACCCCA21630.11592664946257306No Hit
CTATTATAGATGCTCGCAATCCACTCCAGTCTCTTCCCTGGGGACTGGCGTACCCAGCCCCAGTGATGACGTCTA21460.11501552924025973No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21190.11356845594599736No Hit
CTATAATAGTGGCAGCATCTATAACAACCCGTCATTCGGGGGTCGATTCACCATGTCCTTGGACACGGACAAGAA20920.11212138265173502No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC20840.11169262019417579No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC20600.11040633282149814No Hit
ACTGTCAACAGAGTCGCAGTCTCTTTTCGCTCGCTTTCGGCGGAGGGACCAAAGTGGAGATTAAACGAACTGTGG20520.10997757036393893No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTCGCAGT20370.1091736407560154No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCCGGAGTCCCTGG20120.10783375807614284No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG19770.10595792232432129No Hit
ATCTATAACAACCCGTCATTCGGGGGTCGATTCACCATGTCCTTGGACACGGACAAGAACCAGTTCTCCCTGCGG19570.10488601618042323No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC19340.10365332411494049No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19280.10333175227177109No Hit
GCTCCTGGGGCTCCTGCTACTTTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCGTCC19210.10295658512140676No Hit
ACATACTTCTGTGTGAGACATCTAAACTACGGTAGCTGGGCCTTTGAATACTACTTTGACTATTGGGGCCAGGGA19200.10290298981421186No Hit
GTCCCTCACCTGCTCTGTCACTGGTGACTCCATCAGTAGTAGACGTCATC19140.10258141797104246No Hit
GTGCCACTGGATCTGGGACAGATTTCACTCTCACCATCATCAGTCTGCAACCTGAAGATTTTGCAACTTACTA19080.10225984612787305No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18780.100651986912026No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18740.10043760568324639No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18680.10011603384007697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA200.00785751850.9003616
GGCGCTA306.8525475E-445.2399183
CTAACGC452.0883745E-645.2399143
CTCTTAT2850.039.3062551
CCTAACG350.001463072438.777072
TCGTATG7050.037.21116640
CTTATAC7400.037.1574481
TATACAC7700.037.0144733
CGTATGC7200.036.95565441
TCTTATA3000.036.191932
GTGGTAT3500.035.88611
ATGCCGT7550.035.86461644
ATCTCGT6550.035.66171337
AATCTCG6550.035.62208636
TTATACA7900.034.788922
ACGAGAC8200.034.37780422
ATATCGT400.002652583434.3506237
CCGTCTT7700.033.95118747
GGCAATC7100.033.71952433
GTATGCC8000.033.3091842
Produced by FastQC (version 0.11.5)