FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865835
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65980.35362183687196347No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63640.34108053498835644No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51550.27628380858971985No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48930.26224183810465557No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44060.23614092350073826No Hit
CCCCTAAACTCCTCATCTATGGTGCATCTAATTTACTAAGTGGGGTCCCT41850.22429636061066494No Hit
GTTTAGGGGCATTCCCTGGCTTTTGTTGATACCAATTTAAATAAGTCTCG41760.2238140028459108No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG38730.2075746247658555No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36690.1966411820980955No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34480.18479661920802215No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33610.18013382748206566No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33160.17772203865829506No Hit
GTTCAGTGCCACTGGATCTGGGACAGATTTCACTCTCACCATCATCAGTCTGCAACCTGAAGATTTTGCAACTTA32260.1728984610107539No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32140.17225531732441507No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31700.1698971238078394No Hit
AGTCAAGACATCGAGACTTATTTAAATTGGTATCAACAAAAGCCAGGGAA31390.1682356692847974No Hit
GTGCAGCCACAGTTCGTTTAATCTCCACTTTGGTCCCTCCGCCGAAAGCG31370.16812847867040762No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29900.16024996851275702No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29850.15998199197678253No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29610.15869570460410487No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAAGACATCGAGACTTATTTAAATTGGTATCAACAAAAG29010.15547998617241074No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28690.15376493634217386No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28080.15049562260328483No Hit
GATTTCACTCTCACCATCATCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTCGCAGTCT27530.14754788070756525No Hit
ACTTACTACTGTCAACAGAGTCGCAGTCTCTTTTCGCTCGCTTTCGGCGG27370.14669035579244682No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC27200.14577923557013348No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTTTGG26410.14154520630173623No Hit
GAAGTATGTAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCCGCAGGGAGAACTGGTTCTTGTCCGTGTCCAAGGA26290.1409020626153974No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26060.13966937054991466No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT25140.13473860228798365No Hit
GGGTACGCCAGTCCCCAGGGAAGAGACTGGAGTGGATTGCGAGCATCTATAATAGTGGCAGCATCTATAACAACC25140.13473860228798365No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG24920.1335595055296958No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGATGATGGTGAGAGTGAAATCTGTCCCAGATCCAGTGGCA24900.13345231491530601No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24660.13216602754262838No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24610.13189805100665386No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT24400.13077254955556092No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24050.12889671380373935No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22890.12267965816913072No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTTTGGCTCCGAGGTGCCAGATGTGACAT22810.1222508957115715No Hit
GTCTTGACTTGCCCGGCAAGTGATGGTGACT22640.12133977548925816No Hit
CCATAGATGAGGAGTTTAGGGGCATTCCCTGGCTTTTGTTGATACCAATT22500.12058944118852953No Hit
CATCTATGGTGCATCTAATTTACTAAGTGGGGTCCCTTCGCGGTTCAGTGCCACTGGATCTGGGACAGATTTCA22240.11919596320146209No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22140.11866001012951306No Hit
CACCATCATCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTCGCAGTCTCTTTTCGCTCG21990.11785608052158952No Hit
GAGTGAAATCTGTCCCAGATCCAGTGGCACTGAACCGCGAAGGGACCCCA21960.11769529460000483No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21680.11619462599854757No Hit
CTATTATAGATGCTCGCAATCCACTCCAGTCTCTTCCCTGGGGACTGGCGTACCCAGCCCCAGTGATGACGTCTA21630.11592664946257306No Hit
CTATAATAGTGGCAGCATCTATAACAACCCGTCATTCGGGGGTCGATTCACCATGTCCTTGGACACGGACAAGAA21520.11533710108342914No Hit
GTTATAGATGCTGCCACTATTATAGATGCTCGCAATCCACTCCAGTCTCTTCCCTGGGGACTGGCGTACCCAGCC21140.11330047941002287No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21070.11292531225965854No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20500.10987037974954912No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20390.10928083137040522No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCACTTTGGTCC20320.10890566422004089No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20270.10863768768406638No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTCGCAGT20250.10853049706967659No Hit
ACTGTCAACAGAGTCGCAGTCTCTTTTCGCTCGCTTTCGGCGGAGGGACCAAAGTGGAGATTAAACGAACTGTGG19960.10697623316102442No Hit
GCTCCTGGGGCTCCTGCTACTTTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCGTCC19950.10692263785382952No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19730.10574354109554168No Hit
ATCTATAACAACCCGTCATTCGGGGGTCGATTCACCATGTCCTTGGACACGGACAAGAACCAGTTCTCCCTGCGG19610.10510039740920284No Hit
GTGCCACTGGATCTGGGACAGATTTCACTCTCACCATCATCAGTCTGCAACCTGAAGATTTTGCAACTTACTA19570.10488601618042323No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19480.10440365841566912No Hit
ACATACTTCTGTGTGAGACATCTAAACTACGGTAGCTGGGCCTTTGAATACTACTTTGACTATTGGGGCCAGGGA19370.10381411003652519No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19200.10290298981421186No Hit
GTCCCTCACCTGCTCTGTCACTGGTGACTCCATCAGTAGTAGACGTCATC19190.10284939450701695No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18820.1008663681408056No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18770.10059839160483108No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCCGGAGTCCCTGG18700.10022322445446677No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18690.10016962914727186No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA405.4320244E-542.52813327
GTGGTAT3850.040.459531
AAGTGAT18750.035.45730270
AATGGTA3600.032.1171270
AACGCAG42350.031.1850596
ATCAACG42000.031.044293
TCAACGC42050.031.008214
CAACGCA42650.030.8069045
TACGAAA950.004803114630.42674470
CGCAGAG44450.029.6355658
AGAGTAC44550.029.41149511
ACGCAGA45050.029.316037
TGTTAGA19800.028.83241570
GCAGAGT46250.028.7010679
CAGAGTA46250.028.48065210
GCTTATA606.0483377E-428.2190551
GGCCGTA500.008406335527.13394722
ATTAGAC756.9947506E-527.09323
GTTCTAC500.00847261327.0902961
TCCTATA3850.026.3901884
Produced by FastQC (version 0.11.5)