FastQCFastQC Report
Wed 8 May 2024
EGAF00004689645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190232
Total Bases87.5 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT44020.36984386237304995No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42720.3589216220031053No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC33970.285406542590016No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG33260.27944131900335395No Hit
GTATGATGACTGGCCTCCCACCTTCGGCCAAGGGACACGACTGGAGATTA31790.267090785661955No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT31640.2658305271577306No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28820.24213766727831212No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTG28390.23852492623286892No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG28220.23709663326141459No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27660.23239166817897688No Hit
GTCATCATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC27010.22693054799400453No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26350.22138541057541722No Hit
GAGTTCACTCTCACCATCAACAGCCTGCAGTCTGAAGATTTTGCAGTTTA26270.22071327270649757No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGC26040.21878087633335352No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAACAGCCTGCAGTCTGAAGATT25940.21794070399720392No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG25060.2105471874390875No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT24790.20827872213148363No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24690.20743854979533402No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT24530.2060942740574947No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23960.20130529174144202No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23880.20063315387252234No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT23530.19769255069599875No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT22980.193071602847176No Hit
GTAATATACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACA22050.1852580001209848No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT21700.18231739694446125No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA21500.18063705227216206No Hit
CTCCTGTATAGTCTCTGGACTCACTTTCAGTGACGCCTGGATGCATTGGG21380.17962884546878255No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21130.17752841462840857No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA20990.17635217335779915No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT20870.17534396655441964No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGGC20570.17282344954597087No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20410.17147917380813152No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC19940.16753036382822845No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19880.1670262604265387No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC18810.15803641642973806No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC18190.1528273479456106No Hit
CCCTTAGACTCTCCTGTATAGTCTCTGGACTCACTTTCAGTGACGCCTGG18110.15215521007669094No Hit
GTTTATTACTGTCAGCAGTATGATGACTGGCCTCCCACCTTCGGCCAAGG18050.15165110667500117No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC17640.14820640009678784No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17580.1477022966950981No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC17240.1448457107521895No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT17050.14324938331350526No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA16470.13837638376383762No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG16460.13829236653022267No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC16410.13787228036214788No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGTTGATGGTGAGAGTGA15950.13400748761585976No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA15920.13375543591501488No Hit
GTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAAAAACCTGGCCAG15860.13325133251332513No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG15570.13081483273849132No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC15570.13081483273849132No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA14840.12468157468459931No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14600.1226651610778403No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT14380.12081678193831119No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCC14340.12048071300385137No Hit
CTGCTACTCTGGCTCCCAGATATCACTGGAGAAGTCGTGATGACGCAGTC14030.11787617876178763No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT13980.11745609259371283No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC13910.1168679719584081No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT13840.1162798513231034No Hit
TCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGC13810.11602779962225852No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13760.11560771345418372No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA13590.11417942048272943No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13520.1135912998474247No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC13080.1098945415683665No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12960.10888633476498699No Hit
TCACTGGAGAAGTCGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCT12840.10787812796160749No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC12820.10771009349437757No Hit
CACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGA12760.10720599009268782No Hit
CCTGTATAGTCTCTGGACTCACTTTCAGTGACGCCTGGATGCATTGGGTC12690.10661786945738311No Hit
GCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA12630.10611376605569334No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12470.104769490317854No Hit
CATAATGACCCCCCCAGGAACAGTAATATACGGCTGTGTCCTCGGTTTTC12400.10418136968254929No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG12360.10384530074808945No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12350.10376128351447449No Hit
CACCATCAACAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGC12290.10325718011278473No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT12250.1029211111783249No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG12210.10258504224386507No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12180.10233299054302018No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC12090.10157683544048556No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGTTGATGGTGAGAGT12020.10098871480518083No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)