FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190232
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73930.6211394081153926No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40550.34068988230865915No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG32000.2688551475678691No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA29360.24667459789351992No Hit
GTATGATGACTGGCCTCCCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT27550.23146747860921232No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26560.2231497724813314No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26370.22155344504264715No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAACAGCCTGCAGTCTGAAGATT24850.20878282553317334No Hit
GAGTTCACTCTCACCATCAACAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATGATGACTGG24450.205422136188575No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23350.19618024049092947No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA23350.19618024049092947No Hit
GTCATCATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGTTGATGGTGAGAGTGAACT23250.19534006815477992No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22760.1912232237076469No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA22350.18777851712943358No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21980.1846698794856801No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCT21940.18433381055122025No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT20320.1707230187055969No Hit
CTCCTGTATAGTCTCTGGACTCACTTTCAGTGACGCCTGGATGCATTGGGTCCGCCAGGTTCCAGGGAAGGGG19870.16694224319292372No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG19070.160220864503727No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT18710.15719624409358846No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18500.15543188218767434No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18100.15207119284307596No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTA17820.14971871030185713No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16910.14207314204289584No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16740.14064484907144154No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGGC16650.1398886939689069No Hit
GTAATATACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACA16560.13913253886637225No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15750.13232714294356057No Hit
CCCTTAGACTCTCCTGTATAGTCTCTGGACTCACTTTCAGTGACGCCTGG15630.1313189361401811No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC15600.1310668844393362No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15600.1310668844393362No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15240.12804226402919766No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15230.1279582467955827No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15220.12787422956196776No Hit
GTTTATTACTGTCAGCAGTATGATGACTGGCCTCCCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA15180.12753816062750792No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15170.12745414339389297No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG15070.12661397105774336No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC14830.12459755745098434No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14780.12417747128290954No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA14650.12308524724591507No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14530.12207704044253558No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14360.12064874747108127No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14140.11880036833155218No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14040.11796019599540257No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT14040.11796019599540257No Hit
GTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG13820.11611181685587348No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC13440.11291916197850504No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12960.10888633476498699No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGTTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA12870.10813017966245235No Hit
CTGCTACTCTGGCTCCCAGATATCACTGGAGAAGTCGTGATGACGCAGTC12870.10813017966245235No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12350.10376128351447449No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12340.10367726628085953No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12280.10317316287916976No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTA200.00793131150.77898828
ACGTACC352.5953515E-548.1922213
TACGTAC405.7200075E-542.1557912
TTAACGG702.3928806E-839.03286435
GTGGTAT3050.038.713411
CGTACCA451.1418909E-437.4891414
ATACGTA451.1438705E-437.47810711
GGTATCA9150.035.0264171
ATATGGG400.002895281733.7416423
GTATCAA18650.032.7410771
GTTCTAA450.00515780429.9875281
GCTTGAC450.00515780429.9875281
TAGTGCT450.00515780429.9875287
CGGTACC952.9557486E-729.3213345
TAACGGC1052.5363988E-829.3158236
AATACGT606.181283E-428.1133110
GCGGTAC1004.7093818E-727.80098744
TGTTAGA9900.027.7081870
CGTGCAA1055.662114E-727.2208957
ATCAACG22600.027.1724743
Produced by FastQC (version 0.11.5)