FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108144
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACTGTGTCTCTGGGCGAGAGGACCACCATCAACTGCAAGTCCAGT39470.3561811461326326No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38330.34589367446830016No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33630.30348041409780674No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24510.2211806407831473No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23830.21504425417635253No Hit
CTCCCTAACTGTGTCTCTGGGCGAGAGGACCACCATCAACTGCAAGTCCAGTCAGAGTCTTTTGTTCAACTCCAA22320.20141786627008765No Hit
ATATTATCTTCCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT21720.19600340749938638No Hit
GTTTATTACTGTCAGCAATATTATCTTCCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA21390.19302545517550065No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20900.18860364717942793No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20260.18282822449067992No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19980.18030147706435265No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19100.1723602708673241No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18130.16360689585469035No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17810.16071918451031633No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17760.1602679796127579No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17480.15774123218643066No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16730.15097315872305406No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC16420.14817568835819173No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA15720.14185881979237355No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC15680.1414978558743268No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15550.14032472314067487No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15540.14023448216116316No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATCTTCCT15350.1385199035504411No Hit
GTCCCTGACCGATTCACTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC15160.13680532493971903No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT14780.13337616771827487No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG14360.12958604657878398No Hit
ACTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCCAAGTAGTTCTTATTGTTGGAGTTGAACAAAAGACT14320.12922508266073723No Hit
GTCTCCAGACTCCCTAACTGTGTCTCTGGGCGAGAGGACCACCATCAACTGCAAGTCCAGTCAGAGTCTTTTGTT14220.12832267286562035No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13240.11947905687347492No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13000.11731327336519443No Hit
CTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT12670.11433532104130871No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12230.11036471794279444No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12200.11009399500425937No Hit
AACCAGGACAGCCTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAA11840.10684531974183861No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11770.1062136328852568No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11620.10486001819258146No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGTAACTTAGGAGGCTGTCCTGGTTTTTGCTGGTA11550.10422833133599965No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11140.10052845117602045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3050.040.7744841
GGTATCA12800.039.4302981
TCGTATG3150.039.41654640
CGCTAAT3300.039.2943832
ACGAGAC3450.039.2783722
GTATCAA32100.038.621561
CACTCGC3450.038.3952128
ACACTCG3450.038.36742827
ACTCGCT3550.038.30682429
TCGCTAA3550.037.42714731
CGAGACA3550.037.22772223
ATCTCGT2850.034.9020737
CCCACGA3800.034.75969719
ATGCCGT3200.034.71121644
GTGGTAT4550.034.245241
TGTTAGA9400.034.11786370
CTCTTAT1600.033.873011
CTAACGC701.1260781E-633.863843
CACGAGA3950.033.44272621
CTCGCTA4150.032.80117430
Produced by FastQC (version 0.11.5)