FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497982
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACTGTGTCTCTGGGCGAGAGGACCACCATCAACTGCAAGTCCAGT69590.46455831912532997No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65610.43798924152626667No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC57610.3845840604226219No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45880.3062787136294028No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43570.2908579675857253No Hit
CTCCCTAACTGTGTCTCTGGGCGAGAGGACCACCATCAACTGCAAGTCCAGTCAGAGTCTTTTGTTCAACTCCAA41000.27370155315617944No Hit
ATATTATCTTCCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT40670.27149858943565414No Hit
GTTTATTACTGTCAGCAATATTATCTTCCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA39590.26428888998666206No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36200.2416584444939926No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG35630.23785332534035789No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG35270.23545009219069385No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35090.2342484756158619No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33720.2251028383518627No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33630.22450203006444672No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33260.22203204043840316No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32350.21595720108786354No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31950.2132869420326813No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA31490.21021614411922174No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC30970.20674480734748482No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATCTTCCT30540.20387427886316392No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29680.1981332218945221No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT29110.1943281027408874No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC28150.18791948100845005No Hit
GTCCCTGACCGATTCACTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC26080.17410089039788196No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25330.16909415466941524No Hit
ACTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCCAAGTAGTTCTTATTGTTGGAGTTGAACAAAAGACT25090.16749199923630592No Hit
AACCAGGACAGCCTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAA24280.16208472464956186No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24270.16201796817318231No Hit
GTCTCCAGACTCCCTAACTGTGTCTCTGGGCGAGAGGACCACCATCAACTGCAAGTCCAGTCAGAGTCTTTTGTT24100.16088310807472986No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24090.1608163515983503No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGTAACTTAGGAGGCTGTCCTGGTTTTTGCTGGTA23280.15540907701160628No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG23270.15534232053522673No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23000.1535398956729787No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT21770.14532884907829333No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTAACTGTGTCTCTGGGCGAGAGGA21670.1446612843144978No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21310.14225805116483375No Hit
GTAAATGAGTAACTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCCAAGTAGTTCTTATTGTTGGAGTTGAA20940.1397880615387902No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20930.13972130506241062No Hit
CTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT20860.13925400972775373No Hit
AGATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC20750.13851968848757862No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20580.13738482838912616No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20400.13618321181429416No Hit
GTCCTGGTTTTTGCTGGTACCAAGCCAAGTAGTTCTTATTGTTGGAGTTG20200.13484808228670303No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19800.1321778232315208No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19740.13177728437324346No Hit
CTACTTGGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAATC19600.1308426937039297No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19430.12970783360547725No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19340.12910702531806123No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT19170.12797216521960877No Hit
CAGTTAGGGAGTCTGGAGACTGGGTCATCACGATGTCCCCGTAGGCACCA19120.127638382837711No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19080.1273713569321928No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18900.12616974035736078No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18690.12476785435339009No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18650.12450082844787187No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATCTTCCTCCTCTCACTTT18190.1214300305344123No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17940.1197611186249234No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17760.11855950205009139No Hit
TTATTACTGTCAGCAATATTATCTTCCTCCTCTCACTTTCGGCGGAGGGA17740.11842598909733226No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATCTTCCTCCTC17650.11782518080991629No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG17580.11735788547525938No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17540.11709085956974116No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17480.11669032071146383No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17450.11649005128232515No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17420.11628978185318649No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17350.11582248651852961No Hit
CTGTGTCTCTGGGCGAGAGGACCACCATCAACTGCAAGTCCAGTCAGAGT17340.11575573004215005No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17200.11482113937283625No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17040.11375303575076336No Hit
CTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAC17020.11361952279800425No Hit
CAATAAGAACTACTTGGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGTTACTCATTTACTGGGCATCTAC16920.1129519580342087No Hit
CTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCCAAGTAGTTCTTAT16790.11208412384127446No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC16760.1118838544121358No Hit
GGACTGAACACAGAGGACTCACCATGGAATTTGGGCTGAGCTGGATTTTC16450.10981440364436956No Hit
GCCTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCACTGGCAGCGGGT16080.10734441401832599No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15980.10667684925453043No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15980.10667684925453043No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15970.10661009277815087No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15940.10640982334901221No Hit
GAACTACTTGGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGTTAC15850.1058090150615962No Hit
ACCATCAACTGCAAGTCCAGTCAGAGTCTTTTGTTCAACTCCAACAATAA15740.1050746938214211No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACC15690.10474091143952331No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTAACTGTGTCTCTGGGCGAGAGGACCACCATCAACTG15600.10414010315210731No Hit
CTTTTGTTCAACTCCAACAATAAGAACTACTTGGCTTGGTACCAGCAAAA15580.10400659019934819No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT15580.10400659019934819No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15560.10387307724658908No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15540.10373956429382995No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15410.10287173010089573No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15370.10260470419537751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12050.044.307131
CGTATGC6950.044.21369641
GTGGTAT3050.043.4836271
ATGCCGT7050.043.3023644
TCGTATG7250.043.28232240
GCCGTCT6500.042.8192746
ATCTCGT6200.042.65830637
TGGTATC3200.042.489592
CTAATCT6450.041.42284834
GTATGCC7350.041.38968742
AATCTCG6550.041.38438436
GTCGTAT503.894318E-641.32103739
GCTAATC6650.041.17813533
ACGAGAC8450.041.0478122
CACGAGA8550.040.96407321
CTTATAC8600.040.728631
CTTGAAA7500.040.6799257
CCGTCTT6950.040.6028747
TATACAC8900.040.1025353
CCCACGA8800.039.79500619
Produced by FastQC (version 0.11.5)