FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497982
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76830.5128900080241284No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT66300.442595438396456No Hit
CCCTAACTGTGTCTCTGGGCGAGAGGACCACCATCAACTGCAAGTCCAGT56870.3796440811705348No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47390.3163589415627157No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42510.2837817810894924No Hit
GTTTATTACTGTCAGCAATATTATCTTCCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA41720.27850801945550746No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41340.27597127335308436No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41200.2750366826837706No Hit
ATATTATCTTCCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT40620.27116480705375634No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA39240.2619524133133776No Hit
CTCCCTAACTGTGTCTCTGGGCGAGAGGACCACCATCAACTGCAAGTCCAGTCAGAGTCTTTTGTTCAACTCCAA34650.23131119065516142No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34330.2291749834110156No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33390.22289987463133737No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32780.21882772957218444No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31900.21295315965078354No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30150.20127077628436124No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATCTTCCT29740.19853376075279944No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29500.1969316053196901No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC29410.1963307970322741No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACCCCG28600.19092352244553004No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28360.18932136701242072No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27800.18558300433516559No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC26570.1773719577404802No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26290.17550277640185263No Hit
GTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACCCCGGATTCCCGGGT26050.17390062096874329No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25310.16896064171665615No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24530.16375363655905079No Hit
AGATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC24330.16241850703145966No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24270.16201796817318231No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG23420.15634366768092006No Hit
ACTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCCAAGTAGTTCTTATTGTTGGAGTTGAACAAAAGACT23400.15621015472816097No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT23110.15427421691315385No Hit
GTCCCTGACCGATTCACTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC22800.15220476614538758No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACC22220.14833289051537335No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21820.1456626314601911No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21680.1447280407908773No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21250.14185751230655644No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGTAACTTAGGAGGCTGTCCTGGTTTTTGCTGGTA21070.1406558957317244No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20850.13918725325137418No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20520.13698428953084885No Hit
GTCCTGGTTTTTGCTGGTACCAAGCCAAGTAGTTCTTATTGTTGGAGTTG20230.13504835171584173No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20170.1346478128575644No Hit
GTCTCCAGACTCCCTAACTGTGTCTCTGGGCGAGAGGACCACCATCAACTGCAAGTCCAGTCAGAGTCTTTTGTT19740.13177728437324346No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT19580.13070918075117058No Hit
GTAAATGAGTAACTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCCAAGTAGTTCTTATTGTTGGAGTTGAA19580.13070918075117058No Hit
CAGTTAGGGAGTCTGGAGACTGGGTCATCACGATGTCCCCGTAGGCACCA19210.12823919112512702No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATCTTCCTCCTC19160.12790540874322923No Hit
AACCAGGACAGCCTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAA19120.127638382837711No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19000.12683730512115635No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18970.12663703569201765No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18630.12436731549511275No Hit
CTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT18520.12363299425493764No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18490.12343272482579898No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTAACTGTGTCTCTGGGCGAGAGGA18260.12189732586906918No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18140.12109624815251452No Hit
TTATTACTGTCAGCAATATTATCTTCCTCCTCTCACTTTCGGCGGAGGGA17990.12009490100682119No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17900.11949409271940518No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17570.11729112899887982No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17010.1135527663216247No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATCTTCCTCCTCTCACTTT16890.11275168860507001No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16850.11248466269955179No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16830.1123511497467927No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16810.11221763679403357No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16630.11101602021920158No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG16600.1108157507900629No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16130.10767819640022376No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15740.1050746938214211No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15680.10467415496314375No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15550.10380632077020951No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15550.10380632077020951No Hit
CTACTTGGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAAGTTACTCATTTACTGGGCATCTACCCGGGAATC15480.10333902543555264No Hit
CTGTGTCTCTGGGCGAGAGGACCACCATCAACTGCAAGTCCAGTCAGAGT15350.1024711912426184No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15340.10240443476623884No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGAGGAGGAAGATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGC15230.10167011352606374No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15130.10100254876226818No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG15080.1006687663803704No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG200.00713402352.15874545
GGTATCA13100.042.9796141
GTGGTAT2850.041.6532481
GACAGGT2700.040.1986921
GTATCAA32750.038.0084761
ACAGGTA2100.037.1478961
TGGTATC3300.034.9442862
ATCAACG38150.032.175383
TCAACGC38300.031.8754924
TATCAAC38950.031.8702032
CAACGCA38800.031.3762765
AACGCAG39050.031.3501286
AACGGGC450.005028635330.1439867
ACGCAGA40650.029.949337
CACCGGG950.005278658629.844270
CGCAGAG41500.029.5810558
AGAGTAC42500.029.4406311
GCAGAGT42650.028.7834429
CAGAGTA43600.028.54517710
TGTTAGA19500.027.26152870
Produced by FastQC (version 0.11.5)