FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179042
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43740.37097915086994354No Hit
GTAATACACAGCCGTGTCCTCAGTTCTCAGGCTGTTCATTTGCAGATACA39230.33272775694165263No Hit
GTGTATTACTGTGCGAAAGCCGAGTATTACTATGGTTTGGGGAATTATTA31800.26971049377375866No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT30550.2591086661883122No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC30150.25571608136096935No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA26610.22569170563898489No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC25710.2180583897774634No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG24770.21008581543320765No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22010.1866769801245418No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCTGACTGGTGCTGCTTGTATATGAGTTGCAGTAATAAT21380.18133365902147675No Hit
GTCCTGGGCCCATTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG21290.1805703274353246No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC20700.17556626481499388No Hit
GGGCACAGGGTCCTGGGCCCATTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC19340.1640314764020281No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC19330.1639466617813445No Hit
GATTATTACTGCAACTCATATACAAGCAGCACCAGTCAGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT19250.16326814481587595No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC18730.1588577845403302No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCTGACTGGTGCTGCTTGTATATGAGTTG17670.14986743474787156No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAACTCATAT17580.14910410316171943No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT17510.14851040081693442No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17110.14511781598959156No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT17010.14426966978275585No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16820.14265819198976795No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG16780.14231893350703367No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAGCTAC16290.13816301709353865No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16230.13765412936943722No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16230.13765412936943722No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC16210.13748450012807006No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAACA16060.1362122808178165No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCTGACTGGTG15990.1356185784730315No Hit
GTATTACTGTGCGAAAGCCGAGTATTACTATGGTTTGGGGAATTATTATCCTCCCGGCTACTGGGGCCAGGGAAC15850.1344311737834615No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15840.1343463591627779No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCGGTAGCTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG15410.1306993304733843No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15110.12815489185287718No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15020.12739156026672502No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG14760.12518638012895214No Hit
CAGTAATACACAGCCGTGTCCTCAGTTCTCAGGCTGTTCATTTGCAGATA14220.12060639061203927No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCTGACTGGTGCTGC14200.12043676137067214No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG14140.11992787364657068No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCATTAAT13600.11534788412965781No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT13480.11433010868145495No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG13140.11144641157821351No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG13070.11085270923342849No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13000.11025900688864348No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12740.10805382675087062No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12720.10788419750950348No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC12650.10729049516471849No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12620.10703605130266776No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12520.10618790509583204No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12190.10338902261327416No Hit
CTCATATACAAGCAGCACCAGTCAGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAA12060.10228643254438774No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCATTCTGCCCTGACTCAGC11980.10160791557891914No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC11930.1011838424755013No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11840.10042051088934914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGA200.00731440751.8296942
GATAGCG200.00790643750.81933225
TCTGGAG12850.045.69444370
GGTATCA9250.042.4507371
TCTACGT400.001339824339.47585368
GTGGTAT2600.039.058621
TTTGACG350.001480352538.6833510
GTATCAA22300.036.5831571
CCGTTAA400.002843870533.86517316
CAGGGAA14750.032.66328470
TATCAAC25300.032.379032
ATCAACG25650.032.070543
TCAACGC26000.031.7703724
AACGCAG26250.031.338836
CAACGCA26350.031.2185735
TTCATCG553.5184433E-430.95340231
TCTATAC450.00507174630.0908783
ACGCAGA28400.029.323967
CGCAGAG29000.028.7172558
TATAAGA1203.2887328E-928.2090022
Produced by FastQC (version 0.11.5)