FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120708
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT82280.3879836356537534No Hit
GTATAAGAATTTCCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATCAGGCGAACTGTGGCTGCACCAT80300.37864713105245984No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC78680.37100817274231057No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATCAGGCGT74110.34945876565750683No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT70100.3305499861367053No Hit
TTCTTATACTGTTGGCAGTAATAAATTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGACTGAATTCTGTC61380.28943164264009946No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC59110.2787276701931619No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC55710.262695288554577No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC48760.22992321432276389No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG48220.22737689488604748No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48200.22728258675876167No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG46110.21742738745739632No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA45260.21341929204775012No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG44600.21030712384731892No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG43270.20403563338281366No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42230.1991316107639524No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG41490.19564221005437804No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40400.19050241711730234No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGGCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA40370.19036095492637364No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT39990.18856910050794357No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT39390.18573985668936976No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGGCAGA39250.18507969979836922No Hit
GAATTCAGTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAATTTATTACTGCCAACAGTATAAGAATTTC35630.16800992875964066No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT35420.16701969342313983No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG34970.16489776055920946No Hit
CTAATACTCTGCCTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA34760.16390752522270866No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34340.161927054549707No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT33020.15570271814884462No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32880.15504256125784407No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCCTGATTTCCACCTTGGTCCCTTGGCCGAATGTCCACGGGAAATT32760.15447671249412934No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32070.15122308210276944No Hit
CTACTAATACTCTGCCTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA31660.1492897654934107No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA30330.14301827502890543No Hit
GTGCAGCCACAGTTCGCCTGATTTCCACCTTGGTCCCTTGGCCGAATGTC30270.14273535064704806No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC29230.13783132802818682No Hit
GTATTACTGTGTCCGGACGAGAGATGACTACCCAAGGGGATGGCTCGACCCCTGGGGCCAGGGAACCCTGGTCAC29200.13768986583725812No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28940.1364638601825428No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28780.13570939516425645No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA28160.1327858432183969No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28030.13217284039103921No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG27350.12896636406332224No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT27150.12802328279046432No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26810.12642004462660583No Hit
CAGTAATACATGGCGCTGTCCGAGGCCTTCAGGCTGCTCCAATGCAGGTAGGCGGTGTTGATGGACTTGTCGG25860.12194040858053065No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGGCAGAGTATTAGTAG24890.11736646440716969No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC24310.11463152871588168No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT24290.11453722058859589No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC24130.11378275557030954No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC23860.11250959585195133No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC23690.11170797677002209No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT23320.10996327641523491No Hit
ACCTTGATGGGACCCCACTTTGTAAACTAGACGCCTGATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG23300.10986896828794912No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT22940.10817142199680485No Hit
CAGTATAAGAATTTCCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATCAGGCGAACTGTGGCTGCACCAT22840.10769988136037588No Hit
GCCCCTAACCTCCTGATCTATCAGGCGTCTAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT22800.10751126510580428No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT21990.10369178595072966No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG21730.10246578029601434No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG21590.1018056234050138No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21300.10043815555936979No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)